Abstract
Isolation and detection of a specific protein from a complex mixture of proteins using molecular sieves provided by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) is a widely used technique in molecular biology and biochemistry. The detection is achieved usually by running prestained standard markers along with the sample protein mixture. Multicolored standard markers are preferred compared to fluorescent labeled or single colored markers as it allows easy visualization of separation of a specific protein band during SDS-PAGE. In addition, these colored markers show evidence for the transfer of protein bands on the membrane in western blotting technique. This review describes a very simple, inexpensive and easy method of prestaining specific proteins with different colors using a variety of Remazol dyes.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227(5259):680–685
Davis BJ (1964) Disc electrophoresis. II. Method and application to human serum proteins. Ann N Y Acad Sci 121:404–427
Simpson RJ (2010) Rapid coomassie blue staining of protein gels. Cold Spring Harb Protoc 2010(4):pdb prot5413
Handschin UE, Ritschard WJ (1976) Spectrophotometric determination of fluorophor, protein, and fluorophor/protein ratios in fluorescamine and MDPF fluorescent antibody conjugates. Anal Biochem 71(1):143–155
Stephens RE (1975) High-resolution preparative SDS-polyacrylamide gel electrophoresis: fluorescent visualization and electrophoretic elution-concentration of protein bands. Anal Biochem 65(1–2):369–379
Mendez E (1982) Isolation and elution of proteins from sodium dodecyl sulfate-polyacrylamide gels with an intermediate agarose-containing layer in the acrylamide gel. Anal Biochem 126(2):403–408
Kumar TK, Raman B, Rao CM (1995) Fluorescent staining for proteins on polyacrylamide gels with 5-dimethylamino-1-naphthalenesulfonyl chloride (dansyl chloride). J Biochem Biophys Methods 30(1):79–84
Griffith IP (1972) Immediate visualization of proteins in dodecyl sulfate-polyacrylamide gels by prestaining with Remazol dyes. Anal Biochem 46(2):402–412
Parkinson D, Redshaw JD (1984) Visible labeling of proteins for polyacrylamide gel electrophoresis with dabsyl chloride. Anal Biochem 141(1):121–126
Bosshard HF, Datyner A (1977) The use of a new reactive dye for quantitation of prestained proteins on polyacrylamide gels. Anal Biochem 82(2):327–333
Datyner A, Finnimore ED (1973) A prestaining method for the quantitative assay of proteins on polyacrylamide gels. Anal Biochem 55(2):479–491
Sun SM, Hall TC (1974) Application of Remazol dye for isolation of protein subunits by preparative SDS-polyacrylamide gel electrophoresis. Anal Biochem 61(1):237–242
Compton MM, Lapp SA, Pedemonte R (2002) Generation of multicolored, prestained molecular weight markers for gel electrophoresis. Electrophoresis 23(19):3262–3265
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Kumar, G. (2018). Multicolored Prestained Standard Protein Marker Generation Using a Variety of Remazol Dyes for Easy Visualization of Protein Bands During SDS-PAGE. In: Kurien, B., Scofield, R. (eds) Protein Gel Detection and Imaging. Methods in Molecular Biology, vol 1853. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8745-0_3
Download citation
DOI: https://doi.org/10.1007/978-1-4939-8745-0_3
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8744-3
Online ISBN: 978-1-4939-8745-0
eBook Packages: Springer Protocols