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Protein Gel Detection and Imaging pp 191–205Cite as

Direct Immunodetection of Antigens Within the Precast Polyacrylamide Gel

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1853))

Abstract

Western blotting is one of the few basic techniques widely used in the study of proteins in life science research. Despite its prevalence, the procedure has remained practically unchanged for more than 20 years. Although the method is viewed as being error-prone and as requiring excessive hands-on time, it is still widely accepted because it provides sensitive and direct information about the protein characteristics. The process is attractive to researchers because it reduces the investment in instrumentation and set up. Here we describe a procedure that eliminates the transfer step of western blotting and allows for antigen detection directly within the polyacrylamide gel, thus minimizing the investment necessary for setting up western blotting.

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Authors and Affiliations

  1. Thermo Fisher Scientific, Rockford, IL, USA

    Surbhi Desai, Boguslawa R. Dworecki & Marie C. Nlend

Authors
  1. Surbhi Desai
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  2. Boguslawa R. Dworecki
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  3. Marie C. Nlend
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Corresponding author

Correspondence to Surbhi Desai .

Editor information

Editors and Affiliations

  1. Section of Endocrinology and Diabetes, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA

    Biji T. Kurien

  2. Section of Endocrinology and Diabetes, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA

    R. Hal Scofield

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Desai, S., Dworecki, B.R., Nlend, M.C. (2018). Direct Immunodetection of Antigens Within the Precast Polyacrylamide Gel. In: Kurien, B., Scofield, R. (eds) Protein Gel Detection and Imaging. Methods in Molecular Biology, vol 1853. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8745-0_23

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  • DOI: https://doi.org/10.1007/978-1-4939-8745-0_23

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8744-3

  • Online ISBN: 978-1-4939-8745-0

  • eBook Packages: Springer Protocols

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