Abstract
Native polyacrylamide gel electrophoresis (PAGE) and sodium dodecyl sulfate (SDS)–PAGE are among the most frequently applied techniques in protein analysis. Here we describe a fast one-step method for fluorescent visualization of proteins. Following PAGE, gels are soaked in solution of potassium ferricyanide (100 mM) in 1 M NaOH, and are kept in the dark for 30 min. Gels are then transferred to water and scanned. The sensitivity of the method is comparable with standard Coomassie Brilliant Blue staining.
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Acknowledgments
This work was supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia; Grant number OI173017.
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Bogdanović Pristov, J., Spasojević, I. (2018). A One-Step Staining Protocol for In-Gel Fluorescent Visualization of Proteins. In: Kurien, B., Scofield, R. (eds) Protein Gel Detection and Imaging. Methods in Molecular Biology, vol 1853. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8745-0_18
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DOI: https://doi.org/10.1007/978-1-4939-8745-0_18
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