Abstract
Imidazole-zinc reverse stain (ZN stain) is known for high sensitivity, ease of use, and cost-effective feature. ZN stain is compatible to many experiments of which those are proteomics-related in particular. Here, we describe the ZN staining procedures and the subsequent procedures incorporated in detail, along with the improvements of setup in aspects of visualization and documentation for postprocessing ZN stained gel images.
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Acknowledgments
This work was supported by the grants from Catholic Fu-Jen University (9991A02-109931041001-2) and National Science Council of the Republic of China (NSC 99-2627-E-030-001). We thank Dr. Kuo Cheng-Yi for linguistic edition, and Mr. Tsai Tzung-Lin and Dr. Wu Szu-Yu for providing gel images.
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Chen, HM. (2018). Revisit of Imidazole-Zinc Reverse Stain for Protein Polyacrylamide Gel Electrophoresis. In: Kurien, B., Scofield, R. (eds) Protein Gel Detection and Imaging. Methods in Molecular Biology, vol 1853. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8745-0_17
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DOI: https://doi.org/10.1007/978-1-4939-8745-0_17
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