Skip to main content
SpringerLink
Log in
Book cover

Microbial Carotenoids pp 117–126Cite as

Adaptive Laboratory Evolution for Enhanced Carotenoid Production in Microalgae

  • Protocol
  • First Online:

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1852))

Abstract

In order to produce natural pigments with competitive prices, algal strains employed in industrial production need to be improved for increasing the productivity of valuable metabolites, thereby reducing the overall production cost. Adaptive laboratory evolution (ALE) is a traditional method for strain improvement, which has been effectively utilized in bacteria and fungi. With the growing interest in algal biotechnology, attempts have recently been put forward to improve microalgal strains with ALE approach. This chapter describes a stepwise adaptive evolution strategy that enhances carotenoid yield from microalgae.

This is a preview of subscription content, log in via an institution.

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Henríquez V, Escobar C, Galarza J, Gimpel J (2016) Carotenoids in microalgae. In: Stange C (ed) Carotenoids in nature. Springer, Switzerland, pp 219–237

    Chapter  Google Scholar 

  2. Sandmann G (2014) Carotenoids of biotechnological importance. In: Schrader J, Bohlmann J (eds) Biotechnology of isoprenoids. Springer, Switzerland, pp 449–467

    Chapter  Google Scholar 

  3. Kuczynska P, Jemiola-Rzeminska M, Strzalka K (2015) Photosynthetic pigments in diatoms. Mar Drugs 13(9):5847–5881

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Guedes AC, Amaro HM, Malcata FX (2011) Microalgae as sources of carotenoids. Mar Drugs 9(4):625–644

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Winkler JD, Kao KC (2014) Recent advances in the evolutionary engineering of industrial biocatalysts. Genomics 104 ((6):406–411

    Article  CAS  Google Scholar 

  6. Hong K-K (2012) Advancing metabolic engineering through combination of systems biology and adaptive evolution. Chalmers University of Technology, Sweden

    Google Scholar 

  7. Banerjee C, Singh PK, Shukla P (2016) Microalgal bioengineering for sustainable energy development: recent transgenesis and metabolic engineering strategies. Biotechnol J 11(3):303–314

    Article  CAS  PubMed  Google Scholar 

  8. Fu W, Gudmundsson O, Feist AM, Herjolfsson G, Brynjolfsson S, Palsson BØ (2012) Maximizing biomass productivity and cell density of Chlorella vulgaris by using light-emitting diode-based photobioreactor. J Biotechnol 161(3):242–249

    Article  CAS  PubMed  Google Scholar 

  9. Fu W, Guðmundsson Ó, Paglia G, Herjólfsson G, Andrésson ÓS, Palsson BØ, Brynjólfsson S (2013) Enhancement of carotenoid biosynthesis in the green microalga Dunaliella salina with light-emitting diodes and adaptive laboratory evolution. Appl Microbiol Biotechnol 97(6):2395–2403

    Article  CAS  PubMed  Google Scholar 

  10. Wang L, Xue C, Wang L, Zhao Q, Wei W, Sun Y (2016) Strain improvement of Chlorella sp. for phenol biodegradation by adaptive laboratory evolution. Bioresour Technol 205:264–268

    Article  CAS  PubMed  Google Scholar 

  11. Li D, Wang L, Zhao Q, Wei W, Sun Y (2015) Improving high carbon dioxide tolerance and carbon dioxide fixation capability of Chlorella sp. by adaptive laboratory evolution. Bioresour Technol 185:269–275

    Article  CAS  PubMed  Google Scholar 

  12. Dragosits M, Mattanovich D (2013) Adaptive laboratory evolution—principles and applications for biotechnology. Microb Cell Fact 12(1):1

    Article  Google Scholar 

  13. Fu W, Paglia G, Magnúsdóttir M, Steinarsdóttir EA, Gudmundsson S, Palsson BØ, Andrésson ÓS, Brynjólfsson S (2014) Effects of abiotic stressors on lutein production in the green microalga Dunaliella salina. Microb Cell Fact 13(1):3

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Montgomery DC (ed) (2008) Design and analysis of experiments. Response surface methods and designs. John Wiley & Sons, Inc., New Jersey, p 478

    Google Scholar 

  15. Ferreira SC, Bruns R, Ferreira H, Matos G, David J, Brandao G, da Silva EP, Portugal L, Dos Reis P, Souza A (2007) Box-Behnken design: an alternative for the optimization of analytical methods. Anal Chim Acta 597(2):179–186

    Article  CAS  PubMed  Google Scholar 

  16. Takouridis SJ, Tribe DE, Gras SL, Martin GJ (2015) The selective breeding of the freshwater microalga Chlamydomonas reinhardtii for growth in salinity. Bioresour Technol 184:18–22

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgment

This work was supported by Icelandic Technology Development Fund with a grant number of 163922-0611.

Author information

Authors and Affiliations

  1. Center for Systems Biology and Faculty of Industrial Engineering, Mechanical Engineering and Computer Science, School of Engineering and Natural Sciences, University of Iceland, Reykjavík, Iceland

    Yixi Su, Zhiqian Yi, Snædís Huld Björnsdóttir, Sigurdur Brynjolfsson & Weiqi Fu

Authors
  1. Yixi Su
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zhiqian Yi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Snædís Huld Björnsdóttir
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Sigurdur Brynjolfsson
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Weiqi Fu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Weiqi Fu .

Editor information

Editors and Affiliations

  1. Instituto de Biotecnología de León (INBIOTEC), Parque Científico de León, León, Spain

    Carlos Barreiro

  2. Department of Biotechnology, Crystal Pharma, A Subsidiary of Albany, Molecular Research Inc. (AMRI), Parque Tecnológico de León, León, Spain

    José-Luis Barredo

Rights and permissions

Reprints and permissions

Copyright information

© 2018 Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Su, Y., Yi, Z., Björnsdóttir, S.H., Brynjolfsson, S., Fu, W. (2018). Adaptive Laboratory Evolution for Enhanced Carotenoid Production in Microalgae. In: Barreiro, C., Barredo, JL. (eds) Microbial Carotenoids. Methods in Molecular Biology, vol 1852. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8742-9_7

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-8742-9_7

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8741-2

  • Online ISBN: 978-1-4939-8742-9

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation