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Microbial Carotenoids pp 107–115Cite as

Preparative Recovery of Carotenoids from Microalgal Biomass

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1852))

Abstract

Carotenoids are widespread substances with important physiological roles, and some of them, such as lutein, astaxanthin, or vaucherioxanthin, are high-value products that can be used as high-quality food color and antioxidants, and some have an alleged role in the prevention of disorders such as AMD. Carotenoid extracts are currently obtained from plant sources, but microalgae have been demonstrated to be a competitive source likely to become an alternative. The extraction of carotenoids from microalgae possesses specific problems that arise from the different structure and composition of the source biomass. Here is presented a method for the recovery of carotenoid extracts from microalgal biomass in the kilogram scale.

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References

  1. Pulz O, Gross W (2004) Valuable products from biotechnology of microalgae. Appl Microbiol Biotechnol 65(6):635–648

    Article  CAS  PubMed  Google Scholar 

  2. Business Communications Company, (2015) The Global Market for Carotenoids—FOD025E. https://www.bccresearch.com/market-research/food-and-beverage/carotenoids-global-market-fod025d.html

  3. Cerón MC, García-Malea MC, Rivas J, Acién FG, Fernández JM, Del Río E (2007) Antioxidant activity of Haematococcus pluvialis cells grown in continuous culture as a function of their carotenoid and fatty acid content. Appl Microbiol Biotechnol 74:1112e9

    Article  CAS  Google Scholar 

  4. Chew BP, Park JS, Wong MW, Wong TS (1999) A comparison of the anticancer activities of dietary β-carotene, canthaxanthin and astaxanthin in mice in vivo. Anticancer Res 19:1849–1853

    PubMed  CAS  Google Scholar 

  5. Li J, Zhu D, Niu J, Shen S, Wang G (2011) An economic assessment of astaxanthin production by large scale cultivation of Haematococcus pluvialis. Biotechnol Adv 29:568–574

    Article  CAS  PubMed  Google Scholar 

  6. Park JS, Chyun JH, Kim YK, Line LL, Chew BP (2010) Astaxanthin decreased oxidative stress and inflammation and enhanced immune response in humans. Nutr Metab (Lond) 7:18

    Article  CAS  Google Scholar 

  7. Capelli B, Cysewski G (2012) Internal Beauty Pill? Sunscreen in a pill? The world’s best kept health secret: natural astaxanthin. Cyanotech Corporation

    Google Scholar 

  8. Granado F, Olmedilla B, Blanco I (2003) Nutritional and clinical relevance of lutein in human health. Br J Nutr 90:487–502

    Article  CAS  PubMed  Google Scholar 

  9. Demmig-Adams B, Adams WW III (2002) Food and photosynthesis: antioxidants in photosynthesis and human nutrition. Science 298:2149–2153

    Article  CAS  PubMed  Google Scholar 

  10. Dufossé L, Pintea A (2005) Third International Congress on pigments in food (Conference Paper). Food Res Int 38:381–382

    Google Scholar 

  11. Virtamo J, Taylor PR, Kontto J, Männistö S, Utriainen M, Weinstein SJ, Huttunen J, Albanes D (2014) Effects of α-tocopherol and β-carotene supplementation on cancer incidence and mortality: 18-year postintervention follow-up of the alpha-tocopherol, beta-carotene cancer prevention study. Int J Cancer 135:178–185

    Article  CAS  PubMed  Google Scholar 

  12. Camacho-Rodríguez J, Cerón-García MC, Macías-Sánchez MD (2016) Long-term preservation of concentrated Nannochloropsis gaditana cultures for use in aquaculture. J Appl Phycol 28:299

    Article  CAS  Google Scholar 

  13. Fernández-Sevilla JM, Acién Fernández FG, Molina Grima E (2012) Obtaining lutein-rich extract from microalgal biomass at preparative scale. Microbial carotenoids from bacteria and microalgae: methods and protocols. Methods Mol Biol 892:307–312 Springer Science+Business Media, LLC

    Article  CAS  PubMed  Google Scholar 

  14. Gong M, Bassi A (2016) Carotenoids from microalgae: a review of recent developments. Biotechnol Adv 34:1396–1412

    Article  CAS  PubMed  Google Scholar 

  15. Fernández-Sevilla JM, Acién Fernández FG, Molina Grima E (2010) Biotechnological production of lutein and its applications. Appl Microbiol Biotechnol 86:27–40

    Article  CAS  PubMed  Google Scholar 

  16. Hita Peña E, Robles Medina A, Jiménez Callejón MJ, Macías Sánchez MD, Esteban Cerdán L, González Moreno PA, Molina Grima E (2015) Extraction of free fatty acids from wet Nannochloropsis gaditana biomass for biodiesel production. Renew Energy 75:366–373

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Chemical Engineering, University of Almería, Almería, Spain

    María del Carmen Cerón García, Cynthia Victoria González López, José María Fernández Sevilla & Emilio Molina Grima

Authors
  1. María del Carmen Cerón García
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  2. Cynthia Victoria González López
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  3. José María Fernández Sevilla
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  4. Emilio Molina Grima
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Corresponding author

Correspondence to María del Carmen Cerón García .

Editor information

Editors and Affiliations

  1. Instituto de Biotecnología de León (INBIOTEC), Parque Científico de León, León, Spain

    Carlos Barreiro

  2. Department of Biotechnology, Crystal Pharma, A Subsidiary of Albany, Molecular Research Inc. (AMRI), Parque Tecnológico de León, León, Spain

    José-Luis Barredo

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Cerón García, M., González López, C., Fernández Sevilla, J., Molina Grima, E. (2018). Preparative Recovery of Carotenoids from Microalgal Biomass. In: Barreiro, C., Barredo, JL. (eds) Microbial Carotenoids. Methods in Molecular Biology, vol 1852. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8742-9_6

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  • DOI: https://doi.org/10.1007/978-1-4939-8742-9_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8741-2

  • Online ISBN: 978-1-4939-8742-9

  • eBook Packages: Springer Protocols

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