Abstract
Astaxanthin represents a high-value ketocarotenoid that has been gaining great attention from both the science and public communities in recent years. Microalgae are the primary source of natural astaxanthin. Chlorella zofingiensis, a freshwater and oleaginous green microalga, is capable of growing well photoautotrophically, heterotrophically, and mixotrophically for high-density biomass and astaxanthin production. Here we describe the astaxanthin production pipeline using C. zofingiensis as cell factories under different trophic conditions, including strain preparation, inoculation, and cultivation, biomass harvest and dewatering, and astaxanthin extraction, determination, and quantification.
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Acknowledgments
This study was partially supported by a grant from the National Natural Science Foundation of China (Project No.: 31571807) and a start-up grant from the National Youth Thousand Talents Program.
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Liu, J. (2018). Batch Cultivation for Astaxanthin Analysis Using the Green Microalga Chlorella zofingiensis Under Multitrophic Growth Conditions. In: Barreiro, C., Barredo, JL. (eds) Microbial Carotenoids. Methods in Molecular Biology, vol 1852. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8742-9_5
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DOI: https://doi.org/10.1007/978-1-4939-8742-9_5
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