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Purification and Identification of Astaxanthin and Its Novel Derivative Produced by Radio-tolerant Sphingomonas astaxanthinifaciens

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Microbial Carotenoids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1852))

Abstract

The red diketocarotenoid, astaxanthin, exhibits extraordinary health-promoting activities such as antioxidant, anti-inflammatory, antitumor, and immune booster, which may potentially protect against many degenerative diseases such as cancers, heart diseases, and exercise-induced fatigue. These numerous health benefits and consumer interest in natural products have therefore increased the market demand of astaxanthin as a nutraceutical and medicinal ingredient in food, aquaculture feed, and pharmaceutical industries. Consequently, many research efforts have been made to discover novel microbial sources with effective biotechnological production of astaxanthin. Using a rapid screening method based on 16S rRNA gene, and effective HPLC-Diode array-MS methods for carotenoids analysis, we isolated a novel astaxanthin-producing bacterium (strain TDMA-17T) that belongs to the family Sphingomonadaceae (Asker et al., FEMS Microbiol Lett 273:140–148, 2007).

In this chapter, we provide a comprehensive description of the methods used for the analysis and identification of carotenoids produced by strain TDMA-17T. We will also describe the methods of isolation and identification for a novel bacterial carotenoid (an astaxanthin derivative), a major carotenoid that is produced by the novel strain. Finally, the identification methods of the novel strain will be summarized.

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Authors and Affiliations

  1. Food Science and Technology Department, Faculty of Agriculture, Alexandria University, Alexandria, Egypt

    Dalal Asker

  2. Department of Materials Science and Engineering, University of Toronto, Toronto, ON, Canada

    Dalal Asker & Tarek S. Awad

  3. Life Science Research Center, College of Bioresource Sciences, Nihon University, Fujisawa, Japan

    Teruhiko Beppu & Kenji Ueda

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  1. Dalal Asker
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  2. Tarek S. Awad
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  3. Teruhiko Beppu
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  4. Kenji Ueda
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Editors and Affiliations

  1. Instituto de Biotecnología de León (INBIOTEC), Parque Científico de León, León, Spain

    Carlos Barreiro

  2. Department of Biotechnology, Crystal Pharma, A Subsidiary of Albany, Molecular Research Inc. (AMRI), Parque Tecnológico de León, León, Spain

    José-Luis Barredo

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Asker, D., Awad, T.S., Beppu, T., Ueda, K. (2018). Purification and Identification of Astaxanthin and Its Novel Derivative Produced by Radio-tolerant Sphingomonas astaxanthinifaciens. In: Barreiro, C., Barredo, JL. (eds) Microbial Carotenoids. Methods in Molecular Biology, vol 1852. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8742-9_10

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