Abstract
Chinese hamster ovary (CHO) and human embryonic kidney 293 (HEK293) cells are the two most popular mammalian hosts for the production of recombinant proteins. In this chapter the suspension cultivation and transfection of these cells in small-scale disposable bioreactors is described. The TubeSpin bioreactor 50 and TubeSpin bioreactor 600 are designed for the cultivation of suspension cells by orbital shaking and have maximum working volumes of about 15 mL and 400 mL, respectively.
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References
Klöckner W, Büchs J (2012) Advances in shaking technologies. Trends Biotechnol 30:307–314
Klöckner W, Diederichs S, Büchs J (2014) Orbitally shaken single-use bioreactors. Adv Biochem Eng Biotechnol 138:45–60
De Jesus MJ, Girard P, Bourgeois M, Baumgartner G, Jacko B, Amstutz H, Wurm FM (2004) TubeSpin satellites: a fast track approach for process development with animal cells using shaking technology. Biochem Eng J 17:217–223
Monteil DT, Tontodonati G, Chimire S, Baldi L, Hacker DL, Bürki CA, Wurm FM (2013) Disposable 600-mL orbitally shaken bioreactor for mammalian cell cultivation in suspension. Biochem Eng J 76:6–12
Monteil DT, Shen X, Tontodonati G, Baldi L, Hacker DL, Wurm FM (2016) Disposable orbitally shaken TubeSpin bioreactor 600 for Sf9 cell cultivation in suspension. Anal Biochem 15:26–28
Zhu LK, Song BY, Wang ZL, Monteil DT, Shen X, Hacker DL, De Jesus M, Wurm FM (2017) Studies on fluid dynamics of the flow field and gas transfer in orbitally shaken tubes. Biotechnol Prog 33:192–200
Zhu LK, Monteil DT, Wang Y, Song B, Hacker DL, Wurm MJ, Li X, Wang Z, Wurm FM (2018) Fluid dynamics of flow fields in a disposable 600-mL orbitally shaken bioreactor. Biochem Eng J 129:84–95
Baldi L, Hacker DL, Meerschman C, Wurm FM (2012) Large-scale transfection of mammalian cells. Methods Mol Biol 808:13–26
Rajendra Y, Balasubramanian S, Hacker DL (2017) Large-scale transient transfection of Chinese hamster ovary cells in suspension. Methods Mol Biol 1603:45–55
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Hacker, D.L., Durrer, L., Quinche, S. (2018). CHO and HEK293 Cultivation and Transfection in Single-Use Orbitally Shaken Bioreactors. In: Hacker, D. (eds) Recombinant Protein Expression in Mammalian Cells. Methods in Molecular Biology, vol 1850. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8730-6_9
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DOI: https://doi.org/10.1007/978-1-4939-8730-6_9
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-8730-6
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