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Processing a 16S rRNA Sequencing Dataset with the Microbiome Helper Workflow

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Microbiome Analysis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1849))

Abstract

Sequencing microbiome samples has recently become a fast and cost-effective method to taxonomically profile communities. The growing interest in analyzing microbial sequencing data has attracted many new researchers to the field. Here, we present a straightforward bioinformatic pipeline that aims to streamline the processing of 16S rRNA sequencing data. This workflow is part of the larger project called Microbiome Helper (Comeau et al. mSyst 2:e00127-16, 2017), which includes other bioinformatic workflows, tutorials, and scripts available here: https://github.com/mlangill/microbiome_helper/wiki.

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Correspondence to Morgan G. I. Langille .

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Douglas, G.M., Comeau, A.M., Langille, M.G.I. (2018). Processing a 16S rRNA Sequencing Dataset with the Microbiome Helper Workflow. In: Beiko, R., Hsiao, W., Parkinson, J. (eds) Microbiome Analysis. Methods in Molecular Biology, vol 1849. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8728-3_9

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  • DOI: https://doi.org/10.1007/978-1-4939-8728-3_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8726-9

  • Online ISBN: 978-1-4939-8728-3

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