Abstract
Genomics studies in potato and other plants have elucidated a large number of genes involved in a wide array of phenotypes. In particular, recent bioinformatic and genomic analyses of oomycetes and fungi have identified many effectors for which the corresponding host resistance-eliciting receptor remains to be found. Functional testing of host resistance gene candidates can be accomplished by generating whole plant transformants to either overexpress or silence these genes to obtain a visible phenotype. However, this is time consuming. Alternatively, Agrobacterium tumefaciens can be used to transiently express genes in plant tissue to observe phenotypic changes. Wild relatives of potato contain a large amount of genotypic diversity and are an excellent tool to identify important agronomic traits, including resistance to diseases. The methods presented here help to facilitate the screening of wild potato accessions using agroinfiltration.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsSpringer Nature is developing a new tool to find and evaluate Protocols. Learn more
References
Thirumala DK, Edgar H, Sophien K (2007) In planta expression of oomycete and fungal genes. Methods in Molecular Biology Plant–Pathogen Interactions: Methods and Protocols 354:35–43
Du J, Rietman H, Vleeshouwers VGAA (2014) Agroinfiltration and PVX Agroinfection in potato and Nicotiana benthamiana. J Vis Exp 83
Tadeusz W, Anna T, Richard M (2005) Optimization of agrobacterium-mediated transient assays of gene expression in lettuce, tomato and Arabidopsis. Plant Biotechnol J 3:259–273
Qiang C, Huafang L, Jonathan H, Jake S, Kahlin L, Matthew D (2014) Agroinfiltration as an effective and scalable strategy of gene delivery for production of pharmaceutical proteins. Adv Tech Biol Med 1(1). https://doi.org/10.4172/atbm.1000103
Leuzinger K, Dent M, Hurtado J, Stahnke J, Lai H, Zhou X, Chen Q (2013) Efficient Agroinfiltration of plants for high-level transient expression of recombinant proteins. J Vis Exp:77
Vaghchhipawala ZMR, Muthappa SK, Kirankumar SM (2011) Agroinoculation and Agroinfiltration: simple tools for complex gene function analyses. Andy Pereira (ed.), plant reverse genetics: methods and protocols. Methods Mol Biol 678:65–76
Dillen W, De CJ, Kapila J, Zambre M, Van MM, Angenon G (1997) The effect of temperature on Agrobacterium tumefaciens-mediated gene transfer to plants. Plant J 12:1459–1463
Vleeshouwers VG, Rietman H, Krenek P, Champouret N, Young C, Oh SK, Wang M, Bouwmeester K, Vosman B, Visser RG, Jacobsen E, Govers F, Kamoun S, Van der Vossen EAG (2008) Effector genomics accelerates discovery and functional profiling of potato disease resistance and Phytophthora infestans avirulence genes. PLoS One 3:e2875
Gibriel HAY, Thomma BPHJ, Seidl MF (2016) The age of effectors: genome-based discovery and applications. Phytopathology 106:1206–1212
Varden FA, De la Concepcion JC, Maidment JHR, Banfield MJ (2017) Taking the stage: effectors in the spotlight. Curr Opin Plant Biol 38:25–33
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Abdullah, S., Halterman, D. (2018). Methods for Transient Gene Expression in Wild Relatives of Potato. In: Ma, W., Wolpert, T. (eds) Plant Pathogenic Fungi and Oomycetes. Methods in Molecular Biology, vol 1848. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8724-5_11
Download citation
DOI: https://doi.org/10.1007/978-1-4939-8724-5_11
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8723-8
Online ISBN: 978-1-4939-8724-5
eBook Packages: Springer Protocols