Abstract
Characterizing the detailed structure of the mammalian synapse is of crucial importance to understand its mechanisms of function. Here I describe a protocol to study synaptic architecture by cryo-electron tomography (cryo-ET), a powerful electron microscopy technique that enables 3D visualization of unstained, fully hydrated cellular structures at molecular resolution. The protocol focuses on purified synaptic terminals (“synaptosomes”), currently the most suitable preparation to analyze mammalian synaptic architecture by cryo-ET.
The original version of this chapter was revised. A correction to this chapter can be found at https://doi.org/10.1007/978-1-4939-8719-1_19
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Change history
24 October 2018
This book was inadvertently published with the incorrect title as Clathrin-Mediated Endoytosis: Methods and Protocols. This has now been corrected throughout the book to Clathrin-Mediated Endocytosis: Methods and Protocols.
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Acknowledgments
I wish to thank Eri Sakata for the critical reading of the manuscript. R. F.-B. is supported by the FP7 GA ERC-2012-SyG_318987–ToPAG grant from the European Commission.
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Fernández-Busnadiego, R. (2018). Cryo-Electron Tomography of the Mammalian Synapse. In: Swan, L. (eds) Clathrin-Mediated Endocytosis. Methods in Molecular Biology, vol 1847. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8719-1_16
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DOI: https://doi.org/10.1007/978-1-4939-8719-1_16
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