Abstract
The recent development of probes and labeling strategies for multicolor super-resolution imaging in living cells allows cell biologists to follow cellular processes with unprecedented details. Here we describe how to image endocytic events at the plasma membrane of living cells using commercial (Leica, Abberior Instruments) or custom built STED microscopes.
The original version of this chapter was revised. A correction to this chapter can be found at https://doi.org/10.1007/978-1-4939-8719-1_19
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Change history
24 October 2018
This book was inadvertently published with the incorrect title as Clathrin-Mediated Endoytosis: Methods and Protocols. This has now been corrected throughout the book to Clathrin-Mediated Endocytosis: Methods and Protocols.
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Acknowledgments
We thank Stephanie Wood Baguley for generating the plasmids used in this protocol. We thank Dr. Joerg Bewersdorf, Dr. Manuel Jütte, Dr. Edward Allgeyer, and Mark Lessard for critically reading the manuscript. This project was supported by the Wellcome Trust (095927/A/11/Z, 092096) and the G. Harold & Leila Y. Mathers Charitable Foundation.
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Bottanelli, F., Schroeder, L. (2018). Stimulated Emission Depletion (STED) Imaging of Clathrin-Mediated Endocytosis in Living Cells. In: Swan, L. (eds) Clathrin-Mediated Endocytosis. Methods in Molecular Biology, vol 1847. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8719-1_14
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DOI: https://doi.org/10.1007/978-1-4939-8719-1_14
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