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Lymphangiogenesis pp 85–96Cite as

Correlative Fluorescence and Scanning Electron Microscopy to Study Lymphovenous Valve Development

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1846))

Abstract

Lymph collected from throughout the body is exclusively returned to blood circulation via two pairs of bilaterally located lymphovenous valves. Lymphovenous valves share numerous similarities with lymphatic and venous valves and are defective in multiple mouse models of lymphedema or lymphatic dysfunction. Here we describe a protocol that combines the strengths of fluorescence microscopy and scanning electron microscopy to precisely locate and analyze the topography of developing lymphovenous valves at high resolution.

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Acknowledgments

This work is supported by NIH/NHLBI (R01HL131652 and R01HL133216 to RSS), Oklahoma Center for Adult Stem Cell Research (4340) to RSS, NIH/NIGMS COBRE (P20 GM103441 to XG; PI: Dr. Rodger McEver) and American Heart Association (15BGIA25710032 for RSS).

Author information

Authors and Affiliations

  1. Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma, OK, USA

    Xin Geng & R. Sathish Srinivasan

  2. Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma, OK, USA

    R. Sathish Srinivasan

Authors
  1. Xin Geng
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  2. R. Sathish Srinivasan
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Corresponding author

Correspondence to R. Sathish Srinivasan .

Editor information

Editors and Affiliations

  1. Center for Vascular & Developmental Biology, Feinberg Cardiovascular Research Institute, Northwestern University, Chicago, IL, USA

    Guillermo Oliver

  2. Department of Medicine and Cardiovascular Institute, University of Pennsylvania, Philadelphia, PA, USA

    Mark L. Kahn

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Geng, X., Srinivasan, R.S. (2018). Correlative Fluorescence and Scanning Electron Microscopy to Study Lymphovenous Valve Development. In: Oliver, G., Kahn, M. (eds) Lymphangiogenesis. Methods in Molecular Biology, vol 1846. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8712-2_6

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  • DOI: https://doi.org/10.1007/978-1-4939-8712-2_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8711-5

  • Online ISBN: 978-1-4939-8712-2

  • eBook Packages: Springer Protocols

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