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Development of Methods for Selective Gene Expression Profiling in Tertiary Lymphoid Structure Using Laser Capture Microdissection

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Tertiary Lymphoid Structures

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1845))

Abstract

Tertiary lymphoid structures (TLS) are de novo lymphoid formations that are induced within tissues during inflammatory episodes. TLS have been reported at various anatomic sites and in many different contexts like cancer, infections, autoimmunity, graft rejection, and idiopathic diseases. These inducible, ectopic, and transient lymphoid structures exhibit the prototypical architecture found within secondary lymphoid organs (SLO) and have been recently appreciated as a major driver of the local adaptive immune reaction. As TLS emerge within tissues, the isolation in situ and the molecular characterization of these structures are challenging to operate. Laser capture microdissection (LCM) is a powerful tool to isolate selective structural components and cells from frozen or paraffin-embedded tissues. We and other groups previously applied LCM to decipher the molecular network within TLS and uncover their intrinsic connection with the local microenvironment. In this chapter, we describe a detailed LCM method for selecting and isolating TLS in situ to perform comprehensive downstream molecular analyses.

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Acknowledgments

We thank all present and past members of the Laboratory “Cancer, Immune control and Escape” (UMRS1138 INSERM, Cordeliers Research Center), pathologists, and clinicians who have participated in these studies, for their help and valuable discussions. We are grateful to lung cancer patients involved in this research performed in our laboratory.

Funding: This work was supported by the Institut National de la Santé et de la Recherche Médicale (INSERM), Pierre and Marie Curie University, Paris-Descartes University, the Labex Immuno-Oncology (LAXE62_9UMRS872 Fridman), CARPEM (Cancer Research for PErsonalized Medicine), Fondation ARC pour la Recherche sur le Cancer. Jeremy Goc was supported by a grant from the Fondation ARC pour la Recherche sur le Cancer.

Author information

Author notes

  1. Claudia Gutierrez-Chavez

    Present address: Department of Radiation Oncology, Albert Einstein College of Medicine, Bronx, NY, USA

  2. Samantha Knockaert

    Present address: Center for Therapeutic Innovation in Oncology, Institut de Recherches Servier, Croissy sur Seine, France

  3. Jérémy Goc

    Present address: Division of Gastroenterology and Hepatology, Joan and Sanford I. Weill Department of Medicine, Weill Cornell Medicine, Cornell University, New York, NY, USA

  4. Jérémy Goc

    Present address: Department of Microbiology and Immunology, Weill Cornell Medicine, Cornell University, New York, NY, USA

  5. Jérémy Goc

    Present address: The Jill Robert’s Institute for Research in Inflammatory Bowel Disease, Weill Cornell Medicine, Cornell University, New York, NY, USA

Authors and Affiliations

  1. Cordeliers Research Center, Laboratory “Cancer, Immune Control and Escape”, Institut National de la Santé et de la Recherche Médicale (INSERM), UMRS 1138, Paris, France

    Claudia Gutierrez-Chavez, Samantha Knockaert, Marie-Caroline Dieu-Nosjean & Jérémy Goc

  2. Cordeliers Research Center, Paris Descartes University, Sorbonne Paris Cité, UMRS 1138, Paris, France

    Claudia Gutierrez-Chavez, Samantha Knockaert, Marie-Caroline Dieu-Nosjean & Jérémy Goc

  3. Cordeliers Research Center, Sorbonne University, UMRS 1138, Paris, France

    Claudia Gutierrez-Chavez, Samantha Knockaert, Marie-Caroline Dieu-Nosjean & Jérémy Goc

  4. Division of Gastroenterology and Hepatology, Joan and Sanford I. Weill Department of Medicine, Weill Cornell Medicine, Cornell University, New York, NY, USA

    Jérémy Goc

  5. Department of Microbiology and Immunology, Weill Cornell Medicine, Cornell University, New York, NY, USA

    Jérémy Goc

  6. The Jill Robert’s Institute for Research in Inflammatory Bowel Disease, Weill Cornell Medicine, Cornell University, New York, NY, USA

    Jérémy Goc

Authors
  1. Claudia Gutierrez-Chavez
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  2. Samantha Knockaert
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  3. Marie-Caroline Dieu-Nosjean
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  4. Jérémy Goc
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Corresponding author

Correspondence to Jérémy Goc .

Editor information

Editors and Affiliations

  1. Laboratory “Cancer, Immune Control and Escape”, UMRS 1138 INSERM, Cordeliers Research Center, Sorbonne University, UMR 1138, Paris Descartes University, Sorbonne Paris City, UMR 1138, Paris, France

    Marie-Caroline Dieu-Nosjean

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Gutierrez-Chavez, C., Knockaert, S., Dieu-Nosjean, MC., Goc, J. (2018). Development of Methods for Selective Gene Expression Profiling in Tertiary Lymphoid Structure Using Laser Capture Microdissection. In: Dieu-Nosjean, MC. (eds) Tertiary Lymphoid Structures. Methods in Molecular Biology, vol 1845. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8709-2_8

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  • DOI: https://doi.org/10.1007/978-1-4939-8709-2_8

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8708-5

  • Online ISBN: 978-1-4939-8709-2

  • eBook Packages: Springer Protocols

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