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Exploring the Role of Tertiary Lymphoid Structures Using a Mouse Model of Bacteria-Infected Lungs

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Tertiary Lymphoid Structures

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1845))

Abstract

Animal models can be helpful tools for deciphering the generation, maintenance, and role of tertiary lymphoid structures (TLS) during infections or tumor development. We describe here the establishment of a persistent lung infection in immune-competent mice by intratracheal instillation of agarose beads containing Pseudomonas aeruginosa or Staphylococcus aureus bacteria. After instillation, animals develop a chronic pulmonary infection, marked by the presence of TLS. This experimental setting allows the study of the function of TLS induced by bacteria encountered in patients with cystic fibrosis (CF) as P. aeruginosa and S. aureus are the two main bacterial strains that infect bronchi of adult CF patients. Additionally, we describe also how to manipulate the immune response in these infected animals by targeting immune cells involved in TLS function. Overall, this approach makes it possible to explore the role of chronic inflammation in the induction and maintenance of TLS in infected tissues.

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Funding

This work was supported by a grant from Association Vaincre la Mucoviscidose (RF201605016 22/1/2/51), by INSERM, and by Paris Descartes and Sorbonne Universities.

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Authors and Affiliations

  1. Cordeliers Research Center, Laboratory “Cancer, immune Control and Escape”, Institut National de la Santé et de la Recherche Médicale (INSERM), UMRS 1138, Paris Cedex 06, France

    Jean-Luc Teillaud & Sophie Sibéril

  2. Cordeliers Research Center, Paris Descartes University, Sorbonne Paris Cité, UMRS 1138, Paris, France

    Jean-Luc Teillaud & Sophie Sibéril

  3. Cordeliers Research Center, Sorbonne University, UMRS 1138, Paris, France

    Jean-Luc Teillaud & Sophie Sibéril

  4. Paris Descartes University, Sorbonne Paris Cité, Faculté de Médecine, UPRES EA 2511, Paris, France

    Lucile Regard, Clémence Martin & Pierre-Régis Burgel

  5. Service de Pneumologie et Service de Physiologie, Hôpital Cochin, AP-HP, Paris, France

    Lucile Regard, Clémence Martin & Pierre-Régis Burgel

Authors
  1. Jean-Luc Teillaud
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  2. Lucile Regard
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  3. Clémence Martin
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  4. Sophie Sibéril
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  5. Pierre-Régis Burgel
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Corresponding author

Correspondence to Jean-Luc Teillaud .

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Editors and Affiliations

  1. Laboratory “Cancer, Immune Control and Escape”, UMRS 1138 INSERM, Cordeliers Research Center, Sorbonne University, UMR 1138, Paris Descartes University, Sorbonne Paris City, UMR 1138, Paris, France

    Marie-Caroline Dieu-Nosjean

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Teillaud, JL., Regard, L., Martin, C., Sibéril, S., Burgel, PR. (2018). Exploring the Role of Tertiary Lymphoid Structures Using a Mouse Model of Bacteria-Infected Lungs. In: Dieu-Nosjean, MC. (eds) Tertiary Lymphoid Structures. Methods in Molecular Biology, vol 1845. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8709-2_13

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  • DOI: https://doi.org/10.1007/978-1-4939-8709-2_13

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8708-5

  • Online ISBN: 978-1-4939-8709-2

  • eBook Packages: Springer Protocols

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