Abstract
Flow cytometry is a powerful technology to assess the presence of NFAT in the nuclei after CRAC channel activation. Here we described a simplified procedure for the analysis of CRAC channel activity using NFAT nuclear translocation by flow cytometry, based on the isolation of Jurkat E6-1 cell nuclei.
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Acknowledgments
This work was supported by grants from Consorcio de Tecnología e Innovación para la Salud, CTI-Salud (CTE-06), Chile (CONICYT 21090900), and INNOVA 12id2-16254.
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Carretta, M.D., Hidalgo, M.A., Burgos, R.A. (2018). Indirect Measurement of CRAC Channel Activity Using NFAT Nuclear Translocation by Flow Cytometry in Jurkat Cells. In: Penna, A., Constantin, B. (eds) The CRAC Channel. Methods in Molecular Biology, vol 1843. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8704-7_7
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DOI: https://doi.org/10.1007/978-1-4939-8704-7_7
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