Abstract
The store-operated calcium entry (SOCE) is the predominant calcium entry mechanism in cancer cell and other non-exciting cells. In the last few years, there is rapidly accumulating evidence supporting that SOCE is dysregulated in many types of cancer. The hyperactive SOCE in tumor cells is spatially and temporally coded to promote cell proliferation, migration, and invasion. In this chapter, we describe two protocols to measure SOCE in tumor cells. The first protocol employs fluorescent microplate readers and could be adapted for high-throughput screening. The second protocol takes advantage of laser scanning confocal microscopy and can be used to resolve the high-resolution spatial and temporal coding of SOCE signals in single cells. These protocols are useful tools to uncover the dysregulation of SOCE signaling in tumor malignancy.
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Acknowledgments
This work was supported by the National Cancer Institute (R01CA175741 to S.Y.) and National Natural Science Foundation of China (31671448, to J.S.)
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Lu, F., Sun, J., Sun, T., Cheng, H., Yang, S. (2018). Fluorescence-Based Measurements of Store-Operated Ca2+ Entry in Cancer Cells Using Fluo-4 and Confocal Live-Cell Imaging. In: Penna, A., Constantin, B. (eds) The CRAC Channel. Methods in Molecular Biology, vol 1843. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8704-7_5
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DOI: https://doi.org/10.1007/978-1-4939-8704-7_5
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