Abstract
Store-operated Ca2+ entry (SOCE) pathway plays important roles in many cellular processes, which is largely studied by using fluorescent Ca2+ indicator, Fura-2. Extracellular Mn2+ is able to cross the plasma membrane through SOCE and quenches the fluorescence signals from Fura-2. Thus, the fluorescence quenching rate by Mn2+ composes a convenient assay to monitor the extent of SOCE. This chapter describes an experimental method of Mn2+ quenching assay for both cultured esophageal epithelial and skeletal muscle cells. It also explains how to perform a quantitative analysis of graded SOCE.
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Acknowledgments
This work was supported by research grants from NIH R01 CA185055 and Pelotonia to ZP.
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Pan, Z., Choi, S., Luo, Y. (2018). Mn2+ Quenching Assay for Store-Operated Calcium Entry. In: Penna, A., Constantin, B. (eds) The CRAC Channel. Methods in Molecular Biology, vol 1843. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8704-7_4
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DOI: https://doi.org/10.1007/978-1-4939-8704-7_4
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