Abstract
Owing to high sample complexity, metaproteomic investigations on bacteria–animal symbioses with two or more uncultured partners can be challenging. A selective isolation or enrichment of distinct (sub-)populations within those consortia can solve this problem. Subsequent discrete proteomic analyses benefit from increased sample purity and higher proteome coverage for each of the individual organisms. Here, we describe centrifugation-based methods that allow for a separation of the host and its bacterial symbiont population(s), or even for an enrichment of distinct symbiotic cell cycle stages in the deep-sea mussels Bathymodiolus azoricus and B. thermophilus, the gutless oligochaete Olavius algarvensis and the deep-sea tube worm Riftia pachyptila, respectively.
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Acknowledgments
We thank Horst Felbeck for valuable help with sample preparation, Ruby Ponnudurai for data collection and analysis, and Thomas Schweder for helpful input. This work was supported by the Deutsche Forschungsgemeinschaft (DFG grant MA 6346/2-1).
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Hinzke, T., Kleiner, M., Markert, S. (2018). Centrifugation-Based Enrichment of Bacterial Cell Populations for Metaproteomic Studies on Bacteria–Invertebrate Symbioses. In: Becher, D. (eds) Microbial Proteomics. Methods in Molecular Biology, vol 1841. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8695-8_22
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DOI: https://doi.org/10.1007/978-1-4939-8695-8_22
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