Abstract
Efficient and reproducible protein enrichment is a prerequisite for the proteomic analysis of highly dilute solutions like culture supernatant or body fluids. Traditionally several precipitation strategies were used for this purpose, but they have certain drawbacks. Here, a protocol for StrataClean enrichment—a very efficient and easy-to-use method for the enrichment of proteins from highly dilute samples—which is compatible to gel-free proteomics, is presented.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Chevallet M, Diemer H, Van Dorssealer A et al (2007) Toward a better analysis of secreted proteins: the example of the myeloid cells secretome. Proteomics 7(11):1757–1770
McCormick RM (1989) A solid-phase extraction procedure for DNA purification. Anal Biochem 181(1):66–74
Zanivan S, Maione F, Hein MY et al (2013) SILAC-based proteomics of human primary endothelial cell morphogenesis unveils tumor Angiogenic markers. Mol Cell Proteomics 12(12):3599–3611
Pasztor L, Ziebandt A, Nega M et al (2010) Staphylococcal major autolysin (Atl) is involved in excretion of cytoplasmic proteins. J Biol Chem 285(47):36794–36803
Bonn F, Bartel J, Büttner K et al (2014) Picking vanished proteins from the void: how to collect and ship/share extremely dilute proteins in a reproducible and highly efficient manner. Anal Chem 86(15):7421–7427
Acknowledgments
The authors like to thank Jürgen Bartel and Knut Büttner for their support in developing of the protocol. This work was supported by grants from the Deutsche Forschungsgemeinschaft (Transregio 34 Pathophysiology of staphylococci in the post-genomic era).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Bonn, F., Otto, A. (2018). Protein Enrichment from Highly Dilute Samples with StrataClean. In: Becher, D. (eds) Microbial Proteomics. Methods in Molecular Biology, vol 1841. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8695-8_2
Download citation
DOI: https://doi.org/10.1007/978-1-4939-8695-8_2
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8693-4
Online ISBN: 978-1-4939-8695-8
eBook Packages: Springer Protocols