Abstract
Vibrio cholerae is an aquatic bacterium that causes the human disease cholera as well as milder forms of diarrhea. V. cholerae is found in the environment in association with a variety of aquatic animals, including vertebrate fish. Here we describe the use of zebrafish (Danio rerio) as a model for the pathogenic life cycle of V. cholerae. Being that fish are natural hosts for V. cholerae, the model provides several significant advantages over existing mammalian models that are not natural hosts.
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References
Allen JP, Neely MN (2010) Trolling for the ideal model host: zebrafish take the bait. Future Microbiol 5(4):563–569
Rowe HM, Withey JH, Neely MN (2014) Zebrafish as a model for zoonotic aquatic pathogens. Dev Comp Immunol 46(1):96–107
Sullivan C, Kim CH (2008) Zebrafish as a model for infectious disease and immune function. Fish Shellfish Immunol 25(4):341–350
Runft DL et al (2014) Zebrafish as a natural host model for Vibrio cholerae colonization and transmission. Appl Environ Microbiol 80(5):1710–1717
Sack DA et al (2004) Cholera. Lancet 363(9404):223–233
Senderovich Y, Izhaki I, Halpern M (2010) Fish as reservoirs and vectors of Vibrio cholerae. PLoS One 5(1):e8607
Traore O et al (2014) Occurrence of Vibrio cholerae in fish and water from a reservoir and a neighboring channel in Ouagadougou, Burkina Faso. J Infect Dev Ctries 8(10):1334–1338
Torres-Vitela MR et al (1997) Incidence of Vibrio cholerae in fresh fish and ceviche in Guadalajara, Mexico. J Food Prot 60(3):237–241
Kiiyukia C et al (1992) Vibrio cholerae non-O1 isolated from ayu fish (Plecoglossus altivelis) in Japan. Appl Environ Microbiol 58(9):3078–3082
Acknowledgments
This work was supported by NIH grants R21AI095520 and R01AI127390 and funding from Wayne State University. We also thank Dr. Melody Neely and her laboratory, who helped in developing this model.
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Mitchell, K.C., Withey, J.H. (2018). Danio rerio as a Native Host Model for Understanding Pathophysiology of Vibrio cholerae . In: Sikora, A. (eds) Vibrio Cholerae. Methods in Molecular Biology, vol 1839. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8685-9_9
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DOI: https://doi.org/10.1007/978-1-4939-8685-9_9
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