Abstract
Chromatin immunoprecipitation (ChIP) measures the physical association between a protein and DNA in the cell. In combination with next-generation sequencing, the technique enables the identification of DNA targets for the corresponding protein across an entire genome. Here we describe the immunoprecipitation of Vibrio cholerae DNA bound to the histone-like nucleoid structuring protein (H-NS) tagged with the Flag epitope. The quality of the DNA obtained in this protocol is suitable for next-generation sequencing. The procedure described herein can be readily adapted to other bacteria and DNA-binding proteins.
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Acknowledgments
This work was supported by Public Service Research Grants and a Ruth L. Kirschstein National Research Service Award from the National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, Maryland, USA.
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Ayala, J.C., Benitez, J.A., Silva, A.J. (2018). Chromatin Immunoprecipitation. In: Sikora, A. (eds) Vibrio Cholerae. Methods in Molecular Biology, vol 1839. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8685-9_7
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DOI: https://doi.org/10.1007/978-1-4939-8685-9_7
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