Metabolomics of Vibrio cholerae

Part of the Methods in Molecular Biology book series (MIMB, volume 1839)


Metabolomics is an “omics” approach to quantitatively measure a large set of metabolites. In this chapter, we describe an example method for performing liquid chromatography coupled to mass spectrometry (LC–MS)-based untargeted metabolomics on a cell extract from Vibrio cholerae.

Key words

Untargeted metabolomics Liquid chromatography–mass spectrometry (LC–MS) Phenyl-bonded phase Reversed-phase chromatography Q-TOF mass spectrometer 



We thank Dr. Fred Stevens for helpful discussions. This research was supported by a grant from the National Institutes of Health to C.C.H. [AI-063121-02].


  1. 1.
    Minato Y et al (2014) Roles of the sodium-translocating NADH:quinone oxidoreductase (Na+-NQR) on vibrio cholerae metabolism, motility and osmotic stress resistance. PLoS One 9:e97083CrossRefPubMedPubMedCentralGoogle Scholar
  2. 2.
    Kirkwood JS, Maier C, Stevens JF (2013) Simultaneous, untargeted metabolic profiling of polar and nonpolar metabolites by LC-Q-TOF mass spectrometry. Curr Protoc Toxicol Chapter 4, Unit 4:39Google Scholar
  3. 3.
    Zhang R et al (2014) Evaluation of mobile phase characteristics on three zwitterionic columns in hydrophilic interaction liquid chromatography mode for liquid chromatography-high resolution mass spectrometry based untargeted metabolite profiling of Leishmania parasites. J Chromatogr A 1362:168–179CrossRefPubMedGoogle Scholar
  4. 4.
    Sheikh KD, Khanna S, Byers SW, Fornace A, Cheema AK (2011) Small molecule metabolite extraction strategy for improving LC/MS detection of cancer cell metabolome. J Biomol Tech 22:1–4PubMedPubMedCentralGoogle Scholar
  5. 5.
    Smith CA, Want EJ, O'Maille G, Abagyan R, Siuzdak G (2006) XCMS: processing mass spectrometry data for metabolite profiling using nonlinear peak alignment, matching, and identification. Anal Chem 78:779–787CrossRefGoogle Scholar
  6. 6.
    Want EJ et al (2006) Solvent-dependent metabolite distribution, clustering, and protein extraction for serum profiling with mass spectrometry. Anal Chem 78:743–752CrossRefPubMedGoogle Scholar
  7. 7.
    Rohmer L, Hocquet D, Miller SI (2011) Are pathogenic bacteria just looking for food? Metabolism and microbial pathogenesis. Trends Microbiol 19:341–348CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Microbiology and ImmunologyUniversity of Minnesota Medical SchoolMinneapolisUSA
  2. 2.Department of PharmaceuticsUniversity of WashingtonSeattleUSA
  3. 3.Department of Biomedical SciencesCollege of Veterinary Medicine, Oregon State UniversityCorvallisUSA

Personalised recommendations