Metabolomics of Vibrio cholerae
Metabolomics is an “omics” approach to quantitatively measure a large set of metabolites. In this chapter, we describe an example method for performing liquid chromatography coupled to mass spectrometry (LC–MS)-based untargeted metabolomics on a cell extract from Vibrio cholerae.
Key wordsUntargeted metabolomics Liquid chromatography–mass spectrometry (LC–MS) Phenyl-bonded phase Reversed-phase chromatography Q-TOF mass spectrometer
We thank Dr. Fred Stevens for helpful discussions. This research was supported by a grant from the National Institutes of Health to C.C.H. [AI-063121-02].
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