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Metabolomics of Vibrio cholerae

  • Yusuke Minato
  • Jay S. Kirkwood
  • Claudia C. HäseEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1839)

Abstract

Metabolomics is an “omics” approach to quantitatively measure a large set of metabolites. In this chapter, we describe an example method for performing liquid chromatography coupled to mass spectrometry (LC–MS)-based untargeted metabolomics on a cell extract from Vibrio cholerae.

Key words

Untargeted metabolomics Liquid chromatography–mass spectrometry (LC–MS) Phenyl-bonded phase Reversed-phase chromatography Q-TOF mass spectrometer 

Notes

Acknowledgments

We thank Dr. Fred Stevens for helpful discussions. This research was supported by a grant from the National Institutes of Health to C.C.H. [AI-063121-02].

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Yusuke Minato
    • 1
  • Jay S. Kirkwood
    • 2
  • Claudia C. Häse
    • 3
    Email author
  1. 1.Department of Microbiology and ImmunologyUniversity of Minnesota Medical SchoolMinneapolisUSA
  2. 2.Department of PharmaceuticsUniversity of WashingtonSeattleUSA
  3. 3.Department of Biomedical SciencesCollege of Veterinary Medicine, Oregon State UniversityCorvallisUSA

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