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Preparation of Vibrio cholerae Samples for RNA-seq Analysis

  • Jyl S. MatsonEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1839)

Abstract

Massively parallel cDNA sequencing (RNA-seq) is a powerful tool for providing an unbiased approach to assess transcript abundance under a variety of conditions. In comparison to microarrays, this technique provides increased resolution and sensitivity and the ability to identify rare transcripts and sRNAs. Here, we describe the sample preparation (based on Illumina technology) used for transcriptomic analysis of V. cholerae cDNA libraries. We describe the entire process from RNA isolation through to the generation of barcoded cDNA libraries ready for sequencing.

Key words

RNA-seq Illumina Next-generation sequencing mRNA enrichment Transcriptomic analysis cDNA synthesis 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Medical Microbiology and ImmunologyUniversity of Toledo College of Medicine and Life SciencesToledoUSA

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