Abstract
Massively parallel cDNA sequencing (RNA-seq) is a powerful tool for providing an unbiased approach to assess transcript abundance under a variety of conditions. In comparison to microarrays, this technique provides increased resolution and sensitivity and the ability to identify rare transcripts and sRNAs. Here, we describe the sample preparation (based on Illumina technology) used for transcriptomic analysis of V. cholerae cDNA libraries. We describe the entire process from RNA isolation through to the generation of barcoded cDNA libraries ready for sequencing.
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Matson, J.S. (2018). Preparation of Vibrio cholerae Samples for RNA-seq Analysis. In: Sikora, A. (eds) Vibrio Cholerae. Methods in Molecular Biology, vol 1839. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8685-9_3
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DOI: https://doi.org/10.1007/978-1-4939-8685-9_3
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8684-2
Online ISBN: 978-1-4939-8685-9
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