Abstract
The democratization of next-generation sequencing (NGS) technologies has enabled scientists to explore the diversity of microbial life in various ecological niches in an unpreceded depth. The role of viruses as a key player in health and disease is becoming increasingly clear. To address the need for an up scalable, reproducible protocol to purify RNA and DNA viruses from a sample, we describe our optimized method. The Novel Enrichment Technique Of Viromes (NetoVIR) allows researchers to attain a fast, reproducible, and high-throughput sample preparation protocol for NGS gut viromics studies. With appropriate prior homogenization steps, this protocol can be extended to any biological samples.
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Acknowledgment
We would like to thank Lila Close for insightful tips and tricks.
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Conceição-Neto, N., Yinda, K.C., Van Ranst, M., Matthijnssens, J. (2018). NetoVIR: Modular Approach to Customize Sample Preparation Procedures for Viral Metagenomics. In: Moya, A., Pérez Brocal, V. (eds) The Human Virome. Methods in Molecular Biology, vol 1838. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8682-8_7
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DOI: https://doi.org/10.1007/978-1-4939-8682-8_7
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Publisher Name: Humana Press, New York, NY
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