Abstract
The availability of otherwise not readily accessible intraocular cells via induced pluripotent stem cell (iPSC) technology offers great potential for disease modelling, drug screening, and cell-based transplantation therapy in degenerative ocular disorders. Direct differentiation of iPSCs into retinal pigment epithelium (RPE) is particularly straightforward, and iPSC-derived RPE cell cultures have been demonstrated to yield pure populations of functional cells that display many features of native RPE. Here, I describe a protocol for the generation of iPSC-derived RPE monolayer, their propagation, and cryostorage. A reliable monitoring for functional cell differentiation is achieved by measuring transepithelial resistance.
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Acknowledgments
I gratefully acknowledge the excellent and dedicated cell culture work of Nico Hertel. This study was in part supported by a grant from Novartis Pharma GmbH, Nürnberg, Germany (Reference No. 3625340) and Institutional Support from Titel 77 (Institute of Human Genetics, University of Regensburg).
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Brandl, C. (2019). Generation of Functional Retinal Pigment Epithelium from Human Induced Pluripotent Stem Cells. In: Weber, B.H.F., Langmann, T. (eds) Retinal Degeneration. Methods in Molecular Biology, vol 1834. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-8669-9_6
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DOI: https://doi.org/10.1007/978-1-4939-8669-9_6
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-8668-2
Online ISBN: 978-1-4939-8669-9
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