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Rho GTPases pp 339–355Cite as

Measuring the Contributions of the Rho Pathway to the DNA Damage Response in Tumor Epithelial Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1821))

Abstract

Actin polymerization, actomyosin ring contraction, and stress fiber formation are examples of relevant actions of the RhoA/B/C pathway as GTPases that regulate the cytoskeleton. However, open questions that remain to be addressed are whether this pathway and/or downstream components protect against or facilitate the formation of DNA double-strand breaks, the most lethal form of DNA damage in cells. Genotoxic drugs are radiomimetic and/or chemotherapeutic agents that are currently used for cancer treatments and are associated with specific methodologies; thus, these compounds should represent good tools to answer these questions. In this chapter, we describe two methods, the alkaline comet assay and homologous/nonhomologous recombination assays, to investigate the mechanism by which the Rho pathway modulates the repair of DNA breaks in tumor epithelial cell lines.

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Author information

Authors and Affiliations

  1. Laboratory of Signaling in Molecular Biosystems, Department of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, Brazil

    Yuli T. Magalhães, Jessica O. Farias, Lucas F. Monteiro & Fábio L. Forti

Authors
  1. Yuli T. Magalhães
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  2. Jessica O. Farias
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  3. Lucas F. Monteiro
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  4. Fábio L. Forti
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Corresponding author

Correspondence to Fábio L. Forti .

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Editors and Affiliations

  1. The Hull York Medical School, University of Hull, Hull, United Kingdom

    Francisco Rivero

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Magalhães, Y.T., Farias, J.O., Monteiro, L.F., Forti, F.L. (2018). Measuring the Contributions of the Rho Pathway to the DNA Damage Response in Tumor Epithelial Cells. In: Rivero, F. (eds) Rho GTPases. Methods in Molecular Biology, vol 1821. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8612-5_23

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  • DOI: https://doi.org/10.1007/978-1-4939-8612-5_23

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8611-8

  • Online ISBN: 978-1-4939-8612-5

  • eBook Packages: Springer Protocols

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