Abstract
The olfactory epithelium is an extremely functionally diversified organ. Scattered distribution of over 1000 different types of olfactory sensory neurons (OSNs) and concha structures of mouse olfactory turbinates have greatly increased technical difficulties in research and limited applicability of certain methods. We have developed a method to monitor intracellular calcium transients of individual OSNs from intact olfactory turbinates. With this method, it becomes feasible to locate OSNs of the same specificity from preparation to preparation based on anatomical landmarks of olfactory turbinates, zonal distribution patterns of OSNs, and neuronal response characteristics. This preparation is steady under perfusion, which largely minimizes artifacts. Since this method does not involve enzymatic digestions or mechanic tearing and chopping, the preparation gives OSNs an environment close to in vivo physiological conditions. This approach has provided a platform for studying interaction between OSNs or modulations of OSN activity by other epithelial cells.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
He J, Ma L, Kim S, Nakai J, Yu CR (2008) Encoding gender and individual information in the mouse vomeronasal organ. Science 320:535–538
Yu CR (2013) Calcium imaging of vomeronasal organ response using slice preparations from transgenic mice expressing G-CaMP2. Methods Mol Biol 1068:211–220
Bolbat A, Schultz C (2017) Recent developments of genetically encoded optical sensors for cell biology. Biol Cell 109:1–23
Nakai J, Ohkura M, Imoto K (2001) A high signal-to-noise Ca(2+) probe composed of a single green fluorescent protein. Nat Biotechnol 19:137–141
Bub G, Tecza M, Helmes M, Lee P, Kohl P (2010) Temporal pixel multiplexing for simultaneous high-speed, high-resolution imaging. Nat Methods 7:209–211
Holy TE (2014) Calcium imaging in populations of olfactory neurons by planar illumination microscopy. Cold Spring Harb Protoc 2014:317–323
Meyer SA, Ozbay BN, Potcoava M, Salcedo E, Restrepo D, Gibson EA (2016) Super-resolution imaging of ciliary microdomains in isolated olfactory sensory neurons using a custom two-color stimulated emission depletion microscope. J Biomed Opt 21:66017
Bootman MD, Rietdorf K, Collins T, Walker S, Sanderson M (2013) Ca2+−sensitive fluorescent dyes and intracellular Ca2+ imaging. Cold Spring Harb Protoc 2013:83–99
Zhang C (2010) Gap junctions in olfactory neurons modulate olfactory sensitivity. BMC Neurosci 11:108
Yu Y, Boyer NP, Zhang C (2014) Three structurally similar odorants trigger distinct signaling pathways in a mouse olfactory neuron. Neuroscience 275:194–210
Yu Y, Zhang C (2014) Purinergic signaling negatively regulates activity of an olfactory receptor in an odorant-dependent manner. Neuroscience 275:89–101
Paredes RM, Etzler JC, Watts LT, Zheng W, Lechleiter JD (2008) Chemical calcium indicators. Methods 46:143–151
Acknowledgments
This work was supported by NIH grant DC04952 to C.Z.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Zhang, C. (2018). Calcium Imaging of Individual Olfactory Sensory Neurons from Intact Olfactory Turbinates. In: Simoes de Souza, F., Antunes, G. (eds) Olfactory Receptors. Methods in Molecular Biology, vol 1820. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8609-5_5
Download citation
DOI: https://doi.org/10.1007/978-1-4939-8609-5_5
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8608-8
Online ISBN: 978-1-4939-8609-5
eBook Packages: Springer Protocols