Abstract
Olfactory receptors (ORs) are the largest family of the G protein-coupled receptors (GPCRs), which are significantly involved in many human diseases and 40% of all drug targets. A platform containing stable and high-quality OR would be a powerful tool for the development of a practical biosensor that can be applied to various applications, such as the early diagnosis of diseases, assessment of food quality, and drug and fragrance development. Significant efforts have been made to develop the biosensor using GPCRs; nevertheless, they remain a challenge. This chapter describes an attractive methodology for the development of a stable bioelectronic nose using OR-embedded nanodiscs. The ORs were produced in Escherichia coli (E. coli), purified with column chromatography, reconstituted into nanodiscs and applied to a carbon nanotube-field effect transistor (CNT-FET) with floating electrodes. The nanodisc-based bioelectronic nose exhibits high-performance in terms of sensitivity, selectivity and stability. This strategy can be used as a practical method for the receptor-based sensing approach, which represents significant progress in nano-bio technology toward a practical biosensor.
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Acknowledgments
This research was supported by the National Research Foundation funded by the Korean government (MSIT) (NRF-2018R1A2B3004498). S.H. acknowledges the support from the BioNano Health-Guard Research Center funded by the Ministry of Science and ICT(MSIT) of Korea as Global Frontier Project (Grant number H-GUARD_2013M3A6B2078961) and from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement No. 682286).
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Yang, H., Lee, M., Kim, D., Hong, S., Park, T.H. (2018). Bioelectronic Nose Using Olfactory Receptor-Embedded Nanodiscs. In: Simoes de Souza, F., Antunes, G. (eds) Olfactory Receptors. Methods in Molecular Biology, vol 1820. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8609-5_18
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DOI: https://doi.org/10.1007/978-1-4939-8609-5_18
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