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In Vivo Two-Photon Imaging of the Olfactory System in Insects

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Olfactory Receptors

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1820))

Abstract

This chapter describes how to apply two-photon neuroimaging to study the insect olfactory system in vivo. It provides a complete protocol for insect brain functional imaging, with some additional remarks on the acquisition of morphological information from the living brain. We discuss the most important choices to make when buying or building a two-photon laser-scanning microscope. We illustrate different possibilities of animal preparation and brain tissue labeling for in vivo imaging. Finally, we give an overview of the main methods of image data processing and analysis, followed by a short description of pioneering applications of this imaging modality.

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Author information

Authors and Affiliations

  1. Department of Biology, University of Konstanz, Konstanz, Germany

    Marco Paoli

  2. Department of Physics, University of Trento, Povo, Italy

    Albrecht Haase

  3. Center for Mind/Brain Sciences, University of Trento, Trento, Italy

    Albrecht Haase

Authors
  1. Marco Paoli
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  2. Albrecht Haase
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Correspondence to Albrecht Haase .

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Editors and Affiliations

  1. Centro de Matemática Computação e Cognição (CMCC), Universidade Federal do ABC, São Bernardo do Campo, São Paulo, Brazil

    Fabio Marques Simoes de Souza

  2. Centro de Matemática Computação e Cognição (CMCC), Universidade Federal do ABC, São Bernardo do Campo, São Paulo, Brazil

    Gabriela Antunes

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Paoli, M., Haase, A. (2018). In Vivo Two-Photon Imaging of the Olfactory System in Insects. In: Simoes de Souza, F., Antunes, G. (eds) Olfactory Receptors. Methods in Molecular Biology, vol 1820. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8609-5_15

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  • DOI: https://doi.org/10.1007/978-1-4939-8609-5_15

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