Abstract
Electrophysiological recording and optical imaging enable the characterization of membrane and odorant response properties of olfactory sensory neurons (OSNs) in the nasal neuroepithelium. Here we describe a method to record the responses of mammalian OSNs to odorant stimulations in an ex vivo preparation of intact olfactory epithelium. The responses of individual OSNs with defined odorant receptor types can be monitored via patch-clamp recording or calcium imaging.
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Acknowledgments
M.M. is supported by the National Institute of Deafness and Other Communication Disorders, National Institute of Health (R01DC006213) and the National Science Foundation (1515930). X.G. is supported by CNRS (ATIP and ATIP-Plus grant), the Conseil Régional Bourgogne-Franche-Comté (PARI grant) and the FEDER (European Funding for Regional Economical Development).
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Grosmaitre, X., Ma, M. (2018). Odor-Induced Electrical and Calcium Signals from Olfactory Sensory Neurons In Situ. In: Simoes de Souza, F., Antunes, G. (eds) Olfactory Receptors. Methods in Molecular Biology, vol 1820. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8609-5_12
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DOI: https://doi.org/10.1007/978-1-4939-8609-5_12
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