Abstract
The method described is for early pronuclear transfer (PNT) in normally fertilized human eggs. The PNT procedure should be performed shortly after the appearance of the two pronuclei. Each pronucleus is pinched off with a minimal amount of surrounding cytoplasm to form a membrane enclosed karyoplast. The karyoplasts are then fused with a fertilized egg which has had its pronuclei removed. An experienced individual will achieve approximately 90% survival of the fertilized eggs manipulated.
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References
Schon EA, DiMauro S, Hirano M (2013) Human mitochondrial DNA: roles of inherited and somatic mutations. Nat Rev Genet 13:878–890
Hyslop LA, Blakeley P et al (2016) Towards clinical application of pronuclear transfer to prevent mitochondrial DNA disease. Nature 534(7607):383–386
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Hyslop, L. (2018). Pronuclear Transfer in Human Oocytes. In: Verlhac, MH., Terret, ME. (eds) Mouse Oocyte Development. Methods in Molecular Biology, vol 1818. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8603-3_4
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DOI: https://doi.org/10.1007/978-1-4939-8603-3_4
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-8603-3
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