Abstract
Bovine mammary organoids are cell aggregates that are produced by an association of a mechanical and an enzymatic dissociation of mammary gland tissue. They provide a useful source to isolate mammary epithelial cells, but can also be frozen as an intermediate dissociation step.
Due to the strong cell–cell interactions among epithelial cells, the production and isolation of organoids is an efficient way to remove unwanted cell population of non-epithelial origin like fibroblasts.
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Martignani, E., Accornero, P., Miretti, S., Baratta, M. (2018). Bovine Mammary Organoids: A Model to Study Epithelial Mammary Cells. In: Baratta, M. (eds) Epithelial Cell Culture. Methods in Molecular Biology, vol 1817. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8600-2_14
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DOI: https://doi.org/10.1007/978-1-4939-8600-2_14
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-8600-2
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