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Methods for the Preparation of an Excised, Cross-Circulated Rat Heart

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Book cover Experimental Models of Cardiovascular Diseases

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1816))

Abstract

The Emax-Pressure-Volume Area (PVA)-VO2 framework proposed by Dr. Suga for canine hearts has dramatically advanced the field of cardiac mechanical work and energetics, i.e., mechanoenergetics. He and his collaborators investigated mechanoenergetics in the left ventricle (LV) of excised, cross-circulated canine heart preparations. We instituted the excised cross-circulated rat whole heart preparations and found a curvilinear end-systolic pressure–volume relation (ESPVR) in the rat LV, in contrast to the linear ESPVR in canine, rabbit, and human LVs. Although Emax, the slope of the linear ESPVR, could be used as an index of LV contractility, it was not applicable for evaluating LV contractility in the rat LV. Thus, we proposed a new index of contractility, equivalent Emax (eEmax) in the rat LV. We also found a linear VO2–PVA relationship in the rat LV. Here, we introduce the methods for the preparation of excised, cross-circulated rat whole hearts and the eEmax-PVA-VO2 framework in the rat LV. Using this method, we can obtain accurate LV volume and myocardial O2 consumption in real time for estimating cardiac mechanoenergetics, which is very challenging in in vivo experiments.

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Acknowledgment

This work was supported in part by grants-in-aid NO. 25460283 for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.

Disclosures: None.

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Authors and Affiliations

  1. Department of Physiology, Gifu University Graduate School of Medicine, Gifu, Japan

    Koji Obata & Miyako Takaki

  2. Department of Orthopaedic Surgery, Nara Medical University School of Medicine, Nara, Japan

    Miyako Takaki

Authors
  1. Koji Obata
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  2. Miyako Takaki
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Corresponding author

Correspondence to Koji Obata .

Editor information

Editors and Affiliations

  1. Icahn School of Medicine at Mount Sinai, New York, New York, USA

    Kiyotake Ishikawa

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Obata, K., Takaki, M. (2018). Methods for the Preparation of an Excised, Cross-Circulated Rat Heart. In: Ishikawa, K. (eds) Experimental Models of Cardiovascular Diseases. Methods in Molecular Biology, vol 1816. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8597-5_9

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  • DOI: https://doi.org/10.1007/978-1-4939-8597-5_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8596-8

  • Online ISBN: 978-1-4939-8597-5

  • eBook Packages: Springer Protocols

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