Abstract
Specific cell lineages differentiated from human induced pluripotent stem cells (iPSCs) are promising sources for cell replacement therapy and are useful biomedical research tools for research on disease mechanisms and drug discovery. Among the different lineages, cardiac lineage has been one of the most efficiently differentiated through several established protocols. In this chapter, we describe our reproducible and highly efficient methodology for differentiating iPSCs into cardiomyocytes using embryoid bodies. We also describe methods to dissociate iPSC-derived cardiomyocytes and to evaluate iPSC-derived cardiomyocytes.
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Acknowledgments
We greatly appreciate colleagues at our laboratories and the Department of Cardiovascular Medicine, Kyoto University. We thank Gordon Keller, Mark Gagliardi, Shunsuke Funakoshi, and Chikako Okubo for providing valuable advice and support for the differentiation method of cardiomyocytes, Ikue Takei, Misato Nishikawa, Azusa Inagaki, and Megumi Narita for technical support, and Peter Karagiannis for proofediting. We would like to express our heartfelt gratitude to Yoko Uematsu for her administrative support.
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Hatani, T., Miki, K., Yoshida, Y. (2018). Induction of Human Induced Pluripotent Stem Cells to Cardiomyocytes Using Embryoid Bodies. In: Ishikawa, K. (eds) Experimental Models of Cardiovascular Diseases. Methods in Molecular Biology, vol 1816. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8597-5_6
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DOI: https://doi.org/10.1007/978-1-4939-8597-5_6
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