Abstract
Increase in cardiac afterload as represented by hypertension is an established risk factor for cardiovascular diseases. Animal models of increased cardiac afterload offer studies aiming at identifying key molecular mechanisms and developing new therapeutic approaches. We have reported that banding of the ascending aorta in pigs results in significant cardiac hypertrophy and increased myocardial fibrosis at the chronic stages. These changes were accompanied by increased stiffness of the heart, but not by systolic dysfunction. In this chapter, we describe methods to surgically band the ascending aorta in pigs. After 3 months, animals develop systolic left ventricular pressure of >200 mmHg with above described changes in the heart.
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References
Thune JJ, Signorovitch J, Kober L, Velazquez EJ, McMurray JJ, Califf RM et al (2008) Effect of antecedent hypertension and follow-up blood pressure on outcomes after high-risk myocardial infarction. Hypertension 51(1):48–54. https://doi.org/10.1161/HYPERTENSIONAHA.107.093682
Toischer K, Rokita AG, Unsold B, Zhu W, Kararigas G, Sossalla S et al (2010) Differential cardiac remodeling in preload versus afterload. Circulation 122(10):993–1003. https://doi.org/10.1161/CIRCULATIONAHA.110.943431
Nolan SE, Mannisi JA, Bush DE, Healy B, Weisman HF (1988) Increased afterload aggravates infarct expansion after acute myocardial infarction. J Am Coll Cardiol 12(5):1318–1325
Ishikawa K, Aguero J, Oh JG, Hammoudi N, Fish LA, Leonardson L et al (2015) Increased stiffness is the major early abnormality in a pig model of severe aortic stenosis and predisposes to congestive heart failure in the absence of systolic dysfunction. J Am Heart Assoc 4(5):e001925. https://doi.org/10.1161/JAHA.115.001925
Motloch LJ, Ishikawa K, Xie C, Hu J, Aguero J, Fish KM et al (2017) Increased afterload following myocardial infarction promotes conduction-dependent arrhythmias that are unmasked by hypokalemia. JACC Basic Transl Sci 2(3):258–269. https://doi.org/10.1016/j.jacbts.2017.02.002
Pfeffer MA, Braunwald E (1990) Ventricular remodeling after myocardial infarction. Experimental observations and clinical implications. Circulation 81(4):1161–1172
Furihata T, Kinugawa S, Takada S, Fukushima A, Takahashi M, Homma T et al (2016) The experimental model of transition from compensated cardiac hypertrophy to failure created by transverse aortic constriction in mice. Int J Cardiol Heart Vasc 11:24–28. https://doi.org/10.1016/j.ijcha.2016.03.007
Acknowledgments
This work is supported by NIH R01 HL139963 (K.I.) and AHA-SDG 17SDG33410873 (K.I.). O.B. was supported by the Deutsche Herzstiftung.
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Bikou, O., Miyashita, S., Ishikawa, K. (2018). Pig Model of Increased Cardiac Afterload Induced by Ascending Aortic Banding. In: Ishikawa, K. (eds) Experimental Models of Cardiovascular Diseases. Methods in Molecular Biology, vol 1816. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8597-5_26
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DOI: https://doi.org/10.1007/978-1-4939-8597-5_26
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