Abstract
A protocol for the elicitation of capsaicinoids, the pungent principle of peppers, as well as for the biosynthetic intermediaries vanillin and ferulic acid was developed for in vitro cell suspension cultures, and immobilized placentas of Capsicum chinense Jacq. in vitro cultures were exposed to different doses of methyl jasmonate and salicylic acid, which were effective in eliciting specialized metabolism in both of these cultures, resulting in an increased accumulation of the analyzed metabolites.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Hartmann T (2007) From waste products to ecochemicals: Fifty years research of plant secondary metabolism. Phytochemistry 68:2831–2846. https://doi.org/10.1016/j.phytochem.2007.09.017
Ramírez-Estrada K, Vidal-Limón H, Hidalgo D et al (2016) Elicitation, an effective strategy of the biotechnological production of bioactive high-added value compounds in plant cell factories. Molecules 21:182. https://doi.org/10.3390/molecules21020182
Guízar-González C, Monforte-González M, Vázquez-Flota F (2016) Yeast extract induction of sanguinarine biosynthesis is partially dependent of the octadecanoic pathway in cell cultures of Argemone mexicana L., the Mexican poppy. Biotechnol Lett 38:1237–1242. https://doi.org/10.1007/s10529-016-2095-2
Vázquez-Flota F, Loyola-Vargas V (2003) In vitro plant cell culture as the basis for the development of a research institute in Mexico: Centro de Investigación Científica de Yucatán. In Vitro Cell Dev-Pl 39:250–258. https://doi.org/10.1079/IVP2002398
Vázquez-Flota F, Hernández-Domínguez E, Miranda-Ham ML, Monforte-González M (2009) A differential response to chemical elicitors in Catharanthus roseus in vitro cultures. Biotechnol Lett 31:591–595. https://doi.org/10.1007/s10529-008-9881-4
Baas-Espinola FM, Castro-Concha LA, Vázquez-Flota F, Miranda-Ham ML (2016) Capsaicin synthesis requires in situ phenylalanine and valine formation in in vitro maintained placentas from Capsicum chinense. Molecules 21:799. https://doi.org/10.3390/molecules21060799
Aldana-Iuit JG, Sauri-Duch E, Miranda-Ham ML, Castro-Concha LA, Cuevas-Glory LF, Vázquez-Flota F (2015) Nitrate promotes capsaicin accumulation in Capsicum chinense immobilized placentas. BioMed Res Intl 2015:1. https://doi.org/10.1155/2738/794084
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol 15:473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Monforte-González M, Medina-Lara F, Gutiérrez-Carbajal MG, Vázquez-Flota F (2007) Capsaicinoid quantitation by in situ densitometry of thin layer chromatography plates. J Liq Chromatogr Relat Technol 30:1697–1704. https://doi.org/10.1080/10826070701225041
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Vázquez-Flota, F.A., de Lourdes Miranda-Ham, M. (2018). Induction of Specialized Metabolism in In Vitro Cultures of Capsicum chinense Jacq. In: Loyola-Vargas, V., Ochoa-Alejo, N. (eds) Plant Cell Culture Protocols. Methods in Molecular Biology, vol 1815. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8594-4_30
Download citation
DOI: https://doi.org/10.1007/978-1-4939-8594-4_30
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8593-7
Online ISBN: 978-1-4939-8594-4
eBook Packages: Springer Protocols