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Elaboration of Transcriptome During the Induction of Somatic Embryogenesis

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Plant Cell Culture Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1815))

Abstract

Somatic embryogenesis (SE) is one of the most studied developmental processes due to its applications, such as plant micropropagation, transformation, and germplasm conservation. The use of massive techniques of sequencing, as well as the use of subtractive hybridization and macroarrays, has led to the identification of hundreds of genes involved in the SE process. These have been important developments to study the molecular aspects of the progress of SE. With the advent of the new massive techniques for sequencing RNA, it has been possible to see a more complete picture of whole processes. In this chapter we present a technique to handle the elaboration of the transcriptome from the extraction of RNA until the assembly of the complete transcriptome.

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Acknowledgment

The work from VMLV laboratory was supported by a grant received from the National Council for Science and Technology (CONACyT, 1515).

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Authors and Affiliations

  1. CONACYT Research Fellow-Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, Mérida, Yucatán, Mexico

    Elsa Góngora-Castillo

  2. CONACYT Research Fellow-Campus de Ciencias Exactas e Ingeniería, Universidad Autónoma de Yucatán, Mérida, Yucatán, Mexico

    Geovanny I. Nic-Can

  3. Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Mérida, Yucatán, Mexico

    Rosa M. Galaz-Ávalos & Víctor M. Loyola-Vargas

Authors
  1. Elsa Góngora-Castillo
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  2. Geovanny I. Nic-Can
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  3. Rosa M. Galaz-Ávalos
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  4. Víctor M. Loyola-Vargas
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Corresponding author

Correspondence to Elsa Góngora-Castillo .

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Editors and Affiliations

  1. Centro de Investigación Científica de Yucatán, Unidad de Bioquímica y Biología, Molecular de Plantas, Mérida, Yucatán, Mexico

    Víctor M. Loyola-Vargas

  2. Departamento de Ingeniería Genética, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Irapuato, Guanajuato, Mexico

    Neftalí Ochoa-Alejo

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Góngora-Castillo, E., Nic-Can, G.I., Galaz-Ávalos, R.M., Loyola-Vargas, V.M. (2018). Elaboration of Transcriptome During the Induction of Somatic Embryogenesis. In: Loyola-Vargas, V., Ochoa-Alejo, N. (eds) Plant Cell Culture Protocols. Methods in Molecular Biology, vol 1815. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8594-4_29

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  • DOI: https://doi.org/10.1007/978-1-4939-8594-4_29

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  • Online ISBN: 978-1-4939-8594-4

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