Abstract
Haploid plants have a gametophytic number of chromosomes (n) in the sporophyte. A doubled haploid (DH) plant results from doubling the chromosome set of a haploid plant, as a consequence a homozygosity plant is produced at every locus (true homozygous plant). DH plants are of great significance in breeding programs for the improvement of plants. Here we describe a protocol for the production of doubled haploid plants in carrot (Daucus carota L.) using parthenogenesis induced by wide pollination.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsSpringer Nature is developing a new tool to find and evaluate Protocols. Learn more
References
Rubatzky VE, Quiros CF, Simon PW (1999) Carrots and related vegetable Umbelliferae. CABI Publ, New York
Simon PW (2000) Domestication, historical development, and modern breeding of carrot. Plant Breed Rev 19:157–190
Forster BP, Heberle-Bors E, Kasha KJ, Touraev A (2007) The resurgence of haploids in higher plants. Trends Plant Sci 12:368–375. https://doi.org/10.1016/j.tplants.2007.06.007
Thomas WTB, Forster B, Gertsson B (2003) Doubled haploids in breeding. In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (eds) Doubled haploids production in crop plants. Kluwer Academic Publisher, Dordrecht, pp 337–349
Adamus A, Michalik B (2003) Anther cultures of carrot (Daucus carota L.). Folia Hort 15:49–58
Staniaszek M, Habdas H (2006) RAPD technique application for intraline evaluation of androgenic carrot plants. Folia Hort 18:87–97
Matsubara S, Dohya N, Murakami K (1995) Callus formation and regeneration of adventitious embryos from carrot, fennel and mitsuba microspores by anther and isolated microspore cultures. Acta Hort 392:129–137
Li J-R, Zhuang F-Y, Ou C-G, Hu H, Zhao Z-W, Mao J-H (2013) Microspore embryogenesis and production of haploid and doubled haploid plants in carrot (Daucus carota L.). Plant Cell Tiss Org 112:275–287. https://doi.org/10.1007/s11240-012-0235-5
Bohanec B, Jakše M, Ihan A, Javornik B (1995) Studies of gynogenesis in onion (Allium cepa L.): induction procedures and genetic analysis of regenerants. Plant Sci 104:215–224. https://doi.org/10.1016/0168-9452(94)04030-K
Lim W, Earle ED (2008) Effect of in vitro and in vivo colchicine treatments on pollen production and fruit set of melon plants obtained by pollination with irradiated pollen. Plant Cell Tiss Org 95:115–124. https://doi.org/10.1007/s11240-008-9422-9
Kantartzi SK, Roupakias G (2009) In vitro gynogenesis in cotton (Gossypium sp.). Plant Cell Tiss Org 96:53–57. https://doi.org/10.1007/s11240-008-9459-9
Kiełkowska A, Adamus A (2010) In vitro culture of unfertilized ovules in carrot (Daucus carota L.). Plant Cell Tiss Org 102:309–319. https://doi.org/10.1007/s11240-010-9735-3
Kiełkowska A, Adamus A, Baranski R (2014) An improved protocol for carrot haploid and doubled haploid plant production using induced parthenogenesis and ovule excision in vitro. In Vitro Cell Dev Biol-Plant 50(3):376–383. https://doi.org/10.1007/s11627-014-9597-1
Brazauskas G, Pasakinskiene I, Jahoor A (2004) AFLP analysis indicates no introgression of maize DNA in wheat x maize crosses. Plant Breed 123:117–121. https://doi.org/10.1046/j.1439-0523.2003.00927.x
Manickam S, Sarkar KR (1999) Foreign pollen tube growth in maize after chemical treatments. Ind J Genet Plant Breed 59:53–58
Monakhova MA, Tyukavin GB, Shmykova NA (1998) Change of ploidy during formation of regenerated plants from androgenous and gynogenous embryos of carrot in vitro. In: Proceedings of the IX international congress on plant tissue and cell culture, plant biotechnology and in vitro biology in the 21st century. Jerusalem, Israel, p 122
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Budahn H, Baranski R, Grzebelus D, Kiełkowska A, Straka P, Metge K, Linke B, Nothnagel T (2014) Mapping genes governing flower architecture and pollen development in a double mutant population of carrot (Daucus carota L.). Front. Plant Sci 5:504. https://doi.org/10.3389/fpls.2014.00504
Rogers SO, Bendich AJ (1988) Extraction of DNA from plant tissues. In: Gelvin SB, Schilperoort RA (eds) Plant molecular biology manual. Kluwer, Academic Publishers, Boston, pp l–10
Grzebelus D, Gladysz M, Baranski R (2015) Gene-specific length polymorphism - a simple tool for routine analysis of homogeneity of carrot (Daucus carota L.) breeding stocks. Acta Hort 1099:691–694. https://doi.org/10.17660/ActaHortic.2015.1099.85
Acknowledgments
The research project was funded by the Polish Ministry of Agriculture and Rural Development (Grant No. HORhn4040 dec – 1/08, 2008-2013).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Kiełkowska, A., Adamus, A., Baranski, R. (2018). Haploid and Doubled Haploid Plant Production in Carrot Using Induced Parthenogenesis and Ovule Excision In Vitro. In: Loyola-Vargas, V., Ochoa-Alejo, N. (eds) Plant Cell Culture Protocols. Methods in Molecular Biology, vol 1815. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8594-4_21
Download citation
DOI: https://doi.org/10.1007/978-1-4939-8594-4_21
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8593-7
Online ISBN: 978-1-4939-8594-4
eBook Packages: Springer Protocols