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Cryopreservation of Pineapple Shoot Tips by the Droplet Vitrification Technique

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Plant Cell Culture Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1815))

Abstract

Cryopreservation is a technique that allows the conservation of many species for long periods. Among the protocols used for cryopreservation, droplet vitrification has shown efficient results in preserving shoot tips of various wild and cultivated pineapple genotypes. The method consists of extraction of shoot tips from plants grown in vitro, dehydration for a period of 48 h in a preculture medium supplemented with a high concentration of sucrose, treatment in a plant vitrification solution (PVS2), and immersion in liquid nitrogen. The method described in this chapter has produced survival and regeneration indices of around 70%, depending on the genotype and physiological conditions of the initial explants. The objective of this chapter is to describe in detail a droplet vitrification protocol for shoot tips that is easy to perform for cryopreservation of pineapple germplasm.

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Acknowledgments

The authors acknowledge Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)/EMBRAPA program, and Embrapa Mandioca e Fruticultura for financial support and Helder Lima Carvalho, technician of the Laboratório de Cultura de Tecido de Plantas, for his valuable collaboration in preparing this chapter.

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Authors and Affiliations

  1. Embrapa Cassava and Fruits, Cruz das Almas, Bahia, Brazil

    Fernanda Vidigal Duarte Souza

  2. Federal University of Recôncavo da Bahia (UFRB), Cruz das Almas, Bahia, Brazil

    Fernanda Vidigal Duarte Souza & Everton Hilo de Souza

  3. Scholarship of Coordination for the Improvement of Higher Education Personnel (CAPES) at CAPES-EMBRAPA Program in Embrapa Cassava and Fruits (CNPMF), Cruz das Almas, Bahia, Brazil

    Everton Hilo de Souza & Lívia de Jesus Vieira

  4. Department of Molecular Biology and Genetics, Faculty of Science Mugla Sitki Kocman University Koteki, Mugla, Turkey

    Ergun Kaya

  5. State University of Feira de Santana (UEFS), Feira de Santana, Bahia, Brazil

    Ronilze Leite da Silva

Authors
  1. Fernanda Vidigal Duarte Souza
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  2. Everton Hilo de Souza
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  3. Ergun Kaya
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  4. Lívia de Jesus Vieira
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  5. Ronilze Leite da Silva
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Corresponding author

Correspondence to Fernanda Vidigal Duarte Souza .

Editor information

Editors and Affiliations

  1. Centro de Investigación Científica de Yucatán, Unidad de Bioquímica y Biología, Molecular de Plantas, Mérida, Yucatán, Mexico

    Víctor M. Loyola-Vargas

  2. Departamento de Ingeniería Genética, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Irapuato, Guanajuato, Mexico

    Neftalí Ochoa-Alejo

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Souza, F.V.D., de Souza, E.H., Kaya, E., de Jesus Vieira, L., da Silva, R.L. (2018). Cryopreservation of Pineapple Shoot Tips by the Droplet Vitrification Technique. In: Loyola-Vargas, V., Ochoa-Alejo, N. (eds) Plant Cell Culture Protocols. Methods in Molecular Biology, vol 1815. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8594-4_18

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  • DOI: https://doi.org/10.1007/978-1-4939-8594-4_18

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8593-7

  • Online ISBN: 978-1-4939-8594-4

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