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Nanoscale Imaging pp 403–414Cite as

Probing Bacterial Adhesion at the Single-Molecule and Single-Cell Levels by AFM-Based Force Spectroscopy

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1814))

Abstract

Functionalization of AFM probes with biomolecules or microorganisms allows for a better understanding of the interaction mechanisms driving microbial adhesion. Here we describe the most commonly used protocols to graft molecules and bacteria to AFM cantilevers. The bioprobes obtained that way enable to measure forces down to the single-cell and single-molecule levels.

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Author information

Authors and Affiliations

  1. Université de Lorraine, CNRS, LCPME, F-54000, Nancy, France

    Sofiane El-Kirat-Chatel

  2. Université de Lorraine, CNRS, LIEC, F-54000, Nancy, France

    Audrey Beaussart

  3. CNRS, Laboratoire de Chimie Physique et Microbiologie pour les Matériaux et l’Environnement, LCPME, UMR7564, Nancy, France

    Sofiane El-Kirat-Chatel

  4. CNRS, LIEC (Laboratoire Interdisciplinaire des Environnements Continentaux), UMR7360, Nancy, France

    Audrey Beaussart

Authors
  1. Sofiane El-Kirat-Chatel
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  2. Audrey Beaussart
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Corresponding author

Correspondence to Sofiane El-Kirat-Chatel .

Editor information

Editors and Affiliations

  1. Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, Nebraska, USA

    Yuri L. Lyubchenko

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El-Kirat-Chatel, S., Beaussart, A. (2018). Probing Bacterial Adhesion at the Single-Molecule and Single-Cell Levels by AFM-Based Force Spectroscopy. In: Lyubchenko, Y. (eds) Nanoscale Imaging. Methods in Molecular Biology, vol 1814. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8591-3_24

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  • DOI: https://doi.org/10.1007/978-1-4939-8591-3_24

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8590-6

  • Online ISBN: 978-1-4939-8591-3

  • eBook Packages: Springer Protocols

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