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Monitoring Expression and Enzyme Activity of Ecto-ARTCs

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ADP-ribosylation and NAD+ Utilizing Enzymes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1813))

Abstract

Mammalian ARTCs are expressed as glycosylphosphatidylinositol (GPI)-anchored ectoenzymes (ARTC1–ARTC4) or secretory proteins (ARTC5) by different cell types. The ARTC2 enzymes catalyze mono-ADP-ribosylation of arginine residues in the extracellular domain of membrane proteins or secretory proteins. In this chapter we provide protocols to monitor the expression and activity of ARTCs on the cell membrane of living cells and in soluble form in biological fluids.

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Author information

Authors and Affiliations

  1. Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany

    Stephan Menzel, Sahil Adriouch, Peter Bannas, Friedrich Haag & Friedrich Koch-Nolte

  2. Faculty of Medicine, University of Rouen, Rouen, France

    Sahil Adriouch

  3. Department of Radiology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany

    Peter Bannas

Authors
  1. Stephan Menzel
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  2. Sahil Adriouch
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  3. Peter Bannas
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  4. Friedrich Haag
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  5. Friedrich Koch-Nolte
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Corresponding author

Correspondence to Friedrich Koch-Nolte .

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Editors and Affiliations

  1. Department of Biology, Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA

    Paul Chang

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Menzel, S., Adriouch, S., Bannas, P., Haag, F., Koch-Nolte, F. (2018). Monitoring Expression and Enzyme Activity of Ecto-ARTCs. In: Chang, P. (eds) ADP-ribosylation and NAD+ Utilizing Enzymes. Methods in Molecular Biology, vol 1813. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-8588-3_11

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  • DOI: https://doi.org/10.1007/978-1-4939-8588-3_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-8587-6

  • Online ISBN: 978-1-4939-8588-3

  • eBook Packages: Springer Protocols

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