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Platelets and Megakaryocytes pp 33–54Cite as

Single-Molecule Localization and Structured Illumination Microscopy of Platelet Proteins

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1812))

Abstract

Superresolution microscopy has become increasingly widespread over the past 5 years and allows users to image biological processes below the diffraction limit of traditional fluorescence microscopy where resolution is restricted to approximately 250 nm. Superresolution refers to a wide range of techniques which employ different approaches to circumvent the diffraction limit. Two of these approaches, structured illumination microscopy (SIM) and single-molecule localization microscopy (SMLM), which provide a doubling and tenfold increase in resolution respectively, are dominating the field. This is partly because of the insights into biology they offer and partly because of their commercialization by the main microscope manufacturers. This chapter provides background to the two techniques, practical considerations for their use, and protocols for their application to platelet biology.

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Authors and Affiliations

  1. Institute of Cardiovascular Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK

    Natalie S. Poulter, Abdullah O. Khan, Chiara Pallini & Steven G. Thomas

  2. COMPARE, University of Birmingham and University of Nottingham, Midlands, UK

    Natalie S. Poulter & Steven G. Thomas

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  1. Natalie S. Poulter
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  2. Abdullah O. Khan
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  3. Chiara Pallini
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  4. Steven G. Thomas
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Correspondence to Steven G. Thomas .

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Editors and Affiliations

  1. Institute for Cardiovascular and Metabolic Research, School of Biological Sciences, University of Reading, Reading, Berkshire, UK

    Jonathan M. Gibbins

  2. Department of Molecular and Cell Biology, University of Leicester, Leicester, UK

    Martyn Mahaut-Smith

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Poulter, N.S., Khan, A.O., Pallini, C., Thomas, S.G. (2018). Single-Molecule Localization and Structured Illumination Microscopy of Platelet Proteins. In: Gibbins, J., Mahaut-Smith, M. (eds) Platelets and Megakaryocytes . Methods in Molecular Biology, vol 1812. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8585-2_3

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  • DOI: https://doi.org/10.1007/978-1-4939-8585-2_3

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