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Three-Dimensional Tissue Models for Studying Ex Vivo Megakaryocytopoiesis and Platelet Production

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Platelets and Megakaryocytes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1812))

Abstract

Three-dimensional (3D) tissue cultures in vitro enable a more physiological reconstruction of native tissues and organs. The bone marrow environment, structure and composition regulate megakaryocyte function and platelet production. Here, we describe the use of silk fibroin protein biomaterials to assemble 3D scaffolds mimicking the bone marrow niche architecture and extracellular matrix composition to support platelet release from human megakaryocytes. Additionally, we also propose the use of hyaluronan hydrogels, functionalized with extracellular matrix components, to reproduce the 3D matrix structure of the bone marrow environment for studying human megakaryocyte function.

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Acknowledgments

This work presented in this chapter was supported by Cariplo Foundation (2010-0807, 2013-0717) and US National Institutes of Health (R01 EB016041-01). Christian A. Di Buduo fellowship was funded by Collegio Ghislieri, Pavia, progetto “Progressi in Biologia e Medicina.” The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Authors and Affiliations

  1. Department of Molecular Medicine, University of Pavia, Pavia, Italy

    Christian A. Di Buduo, Vittorio Abbonante & Alessandra Balduini

  2. Department of Biomedical Engineering, Tufts University, Medford, MA, USA

    Lorenzo Tozzi, David L. Kaplan & Alessandra Balduini

Authors
  1. Christian A. Di Buduo
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  2. Vittorio Abbonante
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  3. Lorenzo Tozzi
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  4. David L. Kaplan
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  5. Alessandra Balduini
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Corresponding author

Correspondence to Alessandra Balduini .

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Editors and Affiliations

  1. Institute for Cardiovascular and Metabolic Research, School of Biological Sciences, University of Reading, Reading, Berkshire, UK

    Jonathan M. Gibbins

  2. Department of Molecular and Cell Biology, University of Leicester, Leicester, UK

    Martyn Mahaut-Smith

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Di Buduo, C.A., Abbonante, V., Tozzi, L., Kaplan, D.L., Balduini, A. (2018). Three-Dimensional Tissue Models for Studying Ex Vivo Megakaryocytopoiesis and Platelet Production. In: Gibbins, J., Mahaut-Smith, M. (eds) Platelets and Megakaryocytes . Methods in Molecular Biology, vol 1812. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8585-2_11

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  • DOI: https://doi.org/10.1007/978-1-4939-8585-2_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8584-5

  • Online ISBN: 978-1-4939-8585-2

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