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DNA Nanotechnology pp 263–278Cite as

DNA Origami Structures Interfaced to Inorganic Nanodevices

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1811))

Abstract

We describe here one way to achieve hybrid DNA–inorganic nanostructures on rigid flat insulating substrates. We report methods to prepare rectangular DNA origami and incubate them onto arrays of anchoring gold nanodots either in a static solution or in a microfluidic system. We give details on the design and lithographic methods employed to pattern usable arrays of gold nanoanchors on naturally oxidized silicon wafer chips. Scanning electron and atomic force microscopy methodological details are also given for obtaining the relevant characterizations of the immobilized and ordered DNA origami.

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Author information

Authors and Affiliations

  1. ENEA, Centro Ricerche Casaccia, Rome, Italy

    Claudia Dalmastri & Piero Morales

  2. Centro NAST, Università di Tor Vergata, Rome, Italy

    Weihua Han, Stefano Vespucci, Liqian Wang & Piero Morales

  3. School of Physical Science and Technology, Lanzhou University, Lanzhou, China

    Weihua Han

  4. Division of Physical Biology, Shanghai Institute of Applied Physics, Shanghai, China

    Liqian Wang

Authors
  1. Claudia Dalmastri
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  2. Weihua Han
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  3. Stefano Vespucci
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  4. Liqian Wang
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  5. Piero Morales
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Corresponding author

Correspondence to Piero Morales .

Editor information

Editors and Affiliations

  1. Department of Pharmacy and Biotechnology, University of Bologna, Bologna, Italy

    Giampaolo Zuccheri

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Dalmastri, C., Han, W., Vespucci, S., Wang, L., Morales, P. (2018). DNA Origami Structures Interfaced to Inorganic Nanodevices. In: Zuccheri, G. (eds) DNA Nanotechnology. Methods in Molecular Biology, vol 1811. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8582-1_18

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  • DOI: https://doi.org/10.1007/978-1-4939-8582-1_18

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8581-4

  • Online ISBN: 978-1-4939-8582-1

  • eBook Packages: Springer Protocols

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