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Spatially Resolved Peptide-DNA Nanoassemblages for Biomarker Detection: A Synergy of DNA-Directed Immobilization and Nanografting

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DNA Nanotechnology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1811))

Abstract

Peptide microarrays are becoming a promising alternative to protein microarrays due to the challenges associated with protein immobilization and purification. Here, we put forward a novel experimental-based approach that combines DNA-directed immobilization, nanografting, and atomic force height measurements to immobilize computationally designed cyclic peptide on an ultra-flat gold substrate. This procedure yields peptide-DNA nanoarrays, which can bind to the solvent-exposed site on the Beta-2-microglobulin (β2m).

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Author information

Authors and Affiliations

  1. Centro di Coordinamento Regionale per le Malattie rare, Azienda Sanitaria Universitaria Integrata di Udine, Udine, Italy

    Abimbola Feyisara Adedeji

  2. School of Food Science and Nutrition, University of Leeds, Leeds, UK

    Abimbola Feyisara Adedeji & Matteo Castronovo

  3. Department of Medical Biochemistry and Biophysics (MBB), Karolinska Institutet, Stockholm, Sweden

    Elena Ambrosetti

  4. Nanoinnovation Lab—Elettra Sincrotrone Trieste, Trieste, Italy

    Elena Ambrosetti & Loredana Casalis

Authors
  1. Abimbola Feyisara Adedeji
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  2. Elena Ambrosetti
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  3. Loredana Casalis
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  4. Matteo Castronovo
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Corresponding author

Correspondence to Matteo Castronovo .

Editor information

Editors and Affiliations

  1. Department of Pharmacy and Biotechnology, University of Bologna, Bologna, Italy

    Giampaolo Zuccheri

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Adedeji, A.F., Ambrosetti, E., Casalis, L., Castronovo, M. (2018). Spatially Resolved Peptide-DNA Nanoassemblages for Biomarker Detection: A Synergy of DNA-Directed Immobilization and Nanografting. In: Zuccheri, G. (eds) DNA Nanotechnology. Methods in Molecular Biology, vol 1811. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8582-1_10

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  • DOI: https://doi.org/10.1007/978-1-4939-8582-1_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8581-4

  • Online ISBN: 978-1-4939-8582-1

  • eBook Packages: Springer Protocols

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