Abstract
A method was developed for the analysis of stimulant drugs, opiates, synthetic opiates, PCP, and benzodiazepines in wastewater samples using liquid chromatography coupled with tandem mass spectrometry (LC-MS-MS). A total of 33 compounds (stimulant-type drugs and metabolites of opiates, synthetic opiates, PCP, and benzodiazepines) were analyzed. These drugs included amphetamine (Amp) (1), methamphetamine (Meth) (2), methylenedioxyamphetamine (MDA) (3), methylenedioxymethamphetamine (MDMA) (4), methylenedioxyethylamphetamine (MDEA) (5), benzoylecgonine (BE, the major metabolite of Coc) (6), cocaine (Coc) (7), 6-monoacetylmorphine (6-MAM, the primary urinary metabolite of heroin) (8), codeine (9), hydrocodone (10), hydromorphone (11), morphine (12), norhydrocodone (the primary urinary metabolite of hydrocodone) (13), oxycodone (14), oxymorphone (15), 2-ethylidine-1,5-dimethyl-3,3-diphenylpyrolidine (EDDP, the primary urinary metabolite of methadone) (16), fentanyl (17), meperidine (18), methadone (19), norfentanyl (the primary urinary metabolite of fentanyl) (20), normeperidine (the primary urinary metabolite of meperidine) (21), phencyclidine (PCP) (22), tramadol (23), alprazolam (24), temazepam (25), nordiazepam (26), chlordiazepoxide (27), flurazepam (28), oxazepam (29), α-OH-alprazolam (the primary urinary metabolite of alprazolam) (30), α-OH-triazolam (the primary urinary metabolite of triazolam) (31), 2-OH-ethylflurazepam (the primary urinary metabolite of flurazepam) (32), and 7-NH2-flunitrazepam (the primary urinary metabolite of flunitrazepam) (33). These drugs were chosen because of their widespread abuse. Wastewater samples were collected at both the Oxford Wastewater Treatment Plant in Oxford, Mississippi (MS), and the University Wastewater Treatment Plant in University, MS. Samples were collected on weekends on which the Ole Miss Rebel football team held home games (Vaught-Hemingway Stadium, University, MS 38677). The collected samples were analyzed using a validated method and found to contain Amp, Meth, MDMA, MDA, Coc, BE, codeine, hydrocodone, hydromorphone, morphine, norhydrocodone, oxycodone, oxymorphone, tramadol, EDDP, meperidine, normeperidine, methadone, alprazolam, α-OH-alprazolam, nordiazepam, oxazepam, and temazepam. None of the samples contained MDEA, 6-MAM, fentanyl, norfentanyl, PCP, chlordiazepoxide, flurazepam, 2-OH-ethylflurazepam, 7-NH2-flunitrazepam, and α-OH-triazolam.
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References
Daughton CG (2001) Illicit drugs in municipal sewage. Proposed new nonintrusive tool to heighten public awareness of societal use of illicit-abused drugs and their potential for ecological consequences. Am Soc Symp Ser 791:348–364
Zuccato E, Chiabrando C, Castiglioni S, Calamari D, Bagnati R, Schiarea S et al (2005) Cocaine in surface waters: a new evidence-based tool to monitor community drug abuse. Environ Health Global Access Sci Source 4:14
Berset JK, Brenneisen R, Mathieu C (2010) Analysis of illicit and illicit drugs in waste, surface, and lake water samples using large volume direct injection high performance liquid chromatography-electrospray tandem mass spectrometry (HPLC-MS/MS). Chemosphere 81:859–866
Huerta-Fontela M, Galceran MT, Ventura F (2007) Ultraperformance liquid chromatography-tandem mass spectrometry analysis of simulatory drugs of abuse in wastewater and surface waters. Anal Chem 79(10):3821–3829
Nuijs ALN, Tarcomnicu I, Bervoets D, Blust R, Jorens PG, Neels H, Corvaci A (2009) Analysis of drugs of abuse in wastewater by hydrophilic interaction liquid chromatography-tandem mass spectrometry. Anal Bioanal Chem 395:819–828
Petrie B, Barden R, Kasprzyk-Hordern B (2015) A review on emerging contaminants in wastewaters and the environment: current knowledge, understudied areas and recommendations for future monitoring. Water Res 72:3–27
Panawennage D, Castiglioni S, Zuccato E, Davoli E, Chiarelli MP (2011) Measurement of illicit drug consumption in small populations: prognosis for noninvasive drug testing for student populations. In: Castiglioni S, Zuccato E, Fanelli R (eds) Illicit drugs in the environment: occurrence, analysis, and fate using mass spectrometry. Chapter 18. Wiley, New Jersey, pp 321–331
Postigo C, López de Alda M, Barceló D (2010) Evaluation of drugs of abuse use and trends in a prison through wastewater analysis. Environ Int 37:49–55
Pal R, Meghraj M, Kirkbride KP, Naidu R (2012) Illicit drugs and the environment—a review. Sci Total Environ 463–464:1079–1092
Mathieu C, Rieckermann J, Berset JD, Schürch S, Brenneisen R (2011) Assessment of total uncertainty in cocaine and benzoylecgonine wastewater load measurements. Water Res 45:6650–6660
Bijlsma L, Sancho J, Pitarch E, Ibáñez M, Hernández F (2009) Simultaneous ultra-high-pressure liquid chromatography-tandem mass spectrometry determination of amphetamine and amphetamine-like stimulants, cocaine and its metabolites, and a cannabis metabolite in surface water and urban wastewater. J Chromatogr A 1216:3078–3089
Gerrity D, Trenholm RA, Snyder SA (2011) Temporal variability of pharmaceuticals and illicit drugs in wastewater and the effects of a major sporting event. Water Res 45:5399–5411
Lai FY, Bruno R, Hall W, Gartner C, Ort C, Kirkbride P et al (2012) Profiles of illicit drug use during annual key holiday and control periods in Australia: wastewater analysis in an urban, a semi-rural and a vacation area. Addiction 108:556–565
Lai FY, Thai PK, O’Brien J, Gartner C, Bruno R, Kele B et al (2013) Using quantitative wastewater analysis to measure daily usage of conventional and emerging illicit drugs at an annual music festival. Drug Alcohol Rev 32:594–602
Hall W, Prichard J, Kirkbride P, Bruno R, Thai PK, Gartner C et al (2012) An analysis of ethical issues in using wastewater analysis to monitor illicit drug use. Addiction 107:1767–1773
Gul W, Stamper BJ, Godfrey M, ElSohly MA (2016) LC-MS/MS method for stimulants in wastewater. J Anal Toxicol 40(2):124–132
Gul W, Stamper BJ, Godfrey M, Gul S, ElSohly MA (2016) LC-MS-MS method for analysis of opiates in wastewater during football games II. J Anal Toxicol 40(5):330–337
Stamper BJ, Gul W, Godfrey M, Gul S, ElSohly MA (2016) LC-MS-MS method for analysis of miscellaneous drugs in wastewater during football games III. J Forensic Toxicol 40(8):694–699
Stamper BJ, Gul W, Godfrey M, Gul S, ElSohly MA (2017) LC-MS-MS method for analysis of benzodiazepines in wastewater during football games IV. J Anal Toxicol 41(3):205–213
Acknowledgements
The authors are thankful to Mr. Randy McCluskey and employees of the Oxford Wastewater Treatment Plant, as well as Mr. David Adkisson and employees of the University Wastewater Treatment Plant for their assistance in collecting samples. The authors are also thankful to Kareem ElSohly, Candice Tolbert, Racheal Speltz, Iram Shahzadi, and Justin Reid (ElSohly Laboratories, Inc.) for their assistance.
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Gul, W., Gul, S.W., Stamper, B., Godfrey, M., ElSohly, M.A. (2018). LC-MS-MS Method Development and Analysis of Stimulants, Opiates, Synthetic Opiates, PCP, and Benzodiazepines in Wastewater. Preponderance of these Drugs During Football Games. In: Musah, R. (eds) Analysis of Drugs of Abuse. Methods in Molecular Biology, vol 1810. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8579-1_15
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DOI: https://doi.org/10.1007/978-1-4939-8579-1_15
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