Abstract
Receptor–receptor interactions that occur in G protein-coupled receptors (GPCRs) oligomers can be explored using three different functional approaches as starting point: (1) quantitative receptor autoradiography (saturation assay); (2) agonist-stimulated [35S]GTPγS binding in autoradiography; and (3) immunohistochemistry. Together, they allow to explore functional changes in receptors signaling transduction, i.e., receptor recognition, G protein activation, and downstream signaling cascades. Here, we describe these three selected methods that have been successfully employed in the study of the functional interaction of dopamine D4 (D4R) and μ opioid (MOR) receptors in the rat caudate putamen in the context of morphine addiction.
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Acknowledgments
This work has been supported by CTS161 (Junta de Andalucía, Spain).
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Rivera, A. et al. (2018). On the Study of D4R-MOR Receptor–Receptor Interaction in the Rat Caudate Putamen: Relevance on Morphine Addiction. In: FUXE, K., Borroto-Escuela, D. (eds) Receptor-Receptor Interactions in the Central Nervous System. Neuromethods, vol 140. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8576-0_3
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DOI: https://doi.org/10.1007/978-1-4939-8576-0_3
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